Biopriming of L. Seeds with a Consortium of Nitrofixing Cyanobacteria Treated with Static Magnetic Field

Overview

Journal Plants (Basel)

Date 2025 Feb 26

PMID 40006887

Authors

Yadenis Ortega Diaz

Liliana Gomez Luna

Yilan Fung Boix

Yadira Silveira Font

Els Prinsen

Michiel Huybrechts

Dries Vandamme

Ann Cuypers

Affiliations

Soon will be listed here.

Abstract

The growing demand for sustainable agriculture necessitates innovative strategies to enhance crop productivity while minimizing environmental impact. This study explores the biopriming potential of L. seeds using extracts derived from a consortium of nitrofixing cyanobacteria , sp., and subjected to static magnetic field (SMF) treatments. The cyanobacterial consortia were exposed to SMF at varying magnetic inductions (40-50 mT and 100-200 mT), followed by extract preparation and application as biopriming agents. Results demonstrated significant improvements in key seedling growth parameters, including root and stem length, vigor index I, and fresh biomass. The consortium treated with 40-50 mT SMF showed the most pronounced growth-stimulating activity, suggesting enhanced bioactive compound production under this treatment that might be related to auxin biosynthesis. Biopriming with cyanobacterial extracts maintained a balanced nutritional uptake and plant health, as indicated by stable fresh weight dry weight ratios. These findings highlight the potential of SMF-enhanced cyanobacterial consortia as biopriming agents for horticultural crops. Future research should elucidate the underlying modes of action and optimize conditions for broader crop applications.

References

Reed R, Bradford K, Khanday I . Seed germination and vigor: ensuring crop sustainability in a changing climate. Heredity (Edinb). 2022; 128(6):450-459. PMC: 9177656. DOI: 10.1038/s41437-022-00497-2. View

Chen H, Ruan J, Chu P, Fu W, Liang Z, Li Y . AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds. Plant J. 2019; 101(2):310-323. DOI: 10.1111/tpj.14542. View

Alvarez X, Alves A, Ribeiro M, Lazzari M, Coutinho P, Otero A . Biochemical characterization of Nostoc sp. exopolysaccharides and evaluation of potential use in wound healing. Carbohydr Polym. 2020; 254:117303. DOI: 10.1016/j.carbpol.2020.117303. View

Parmar P, Kumar R, Neha Y, Srivatsan V . Microalgae as next generation plant growth additives: Functions, applications, challenges and circular bioeconomy based solutions. Front Plant Sci. 2023; 14:1073546. PMC: 10101342. DOI: 10.3389/fpls.2023.1073546. View

Geraskin S, Churyukin R, Volkova P . Radiation exposure of barley seeds can modify the early stages of plants' development. J Environ Radioact. 2017; 177:71-83. DOI: 10.1016/j.jenvrad.2017.06.008. View

Srivastava S, Tyagi R, Sharma S . Seed biopriming as a promising approach for stress tolerance and enhancement of crop productivity: a review. J Sci Food Agric. 2023; 104(3):1244-1257. DOI: 10.1002/jsfa.13048. View

Toribio A, Suarez-Estrella F, Jurado M, Lopez M, Lopez-Gonzalez J, Moreno J . Prospection of cyanobacteria producing bioactive substances and their application as potential phytostimulating agents. Biotechnol Rep (Amst). 2020; 26:e00449. PMC: 7184136. DOI: 10.1016/j.btre.2020.e00449. View

Sarraf M, Deamici K, Taimourya H, Islam M, Kataria S, Raipuria R . Effect of Magnetopriming on Photosynthetic Performance of Plants. Int J Mol Sci. 2021; 22(17). PMC: 8431099. DOI: 10.3390/ijms22179353. View

van Dijk M, Morley T, Rau M, Saghai Y . A meta-analysis of projected global food demand and population at risk of hunger for the period 2010-2050. Nat Food. 2023; 2(7):494-501. DOI: 10.1038/s43016-021-00322-9. View

10.

Haeck A, Van Langenhove H, Harinck L, Kyndt T, Gheysen G, Hofte M . Trace analysis of multi-class phytohormones in Oryza sativa using different scan modes in high-resolution Orbitrap mass spectrometry: method validation, concentration levels, and screening in multiple accessions. Anal Bioanal Chem. 2018; 410(18):4527-4539. DOI: 10.1007/s00216-018-1112-9. View

11.

Singh Y, Kaushal S, Sodhi R . Biogenic synthesis of silver nanoparticles using cyanobacterium sp. WUC 59 cell-free extract and their effects on bacterial growth and seed germination. Nanoscale Adv. 2022; 2(9):3972-3982. PMC: 9417164. DOI: 10.1039/d0na00357c. View

12.

Chen L, Zhang K, Wang M, Zhang Z, Feng Y . Enhancement of magnetic field on fermentative hydrogen production by Clostridium pasteurianum. Bioresour Technol. 2021; 341:125764. DOI: 10.1016/j.biortech.2021.125764. View

13.

Bawa G, Feng L, Shi J, Chen G, Cheng Y, Luo J . Evidence that melatonin promotes soybean seedlings growth from low-temperature stress by mediating plant mineral elements and genes involved in the antioxidant pathway. Funct Plant Biol. 2020; 47(9):815-824. DOI: 10.1071/FP19358. View

14.

Pingali P . Green revolution: impacts, limits, and the path ahead. Proc Natl Acad Sci U S A. 2012; 109(31):12302-8. PMC: 3411969. DOI: 10.1073/pnas.0912953109. View

15.

Singh P, Vaishnav A, Liu H, Xiong C, Singh H, Singh B . Seed biopriming for sustainable agriculture and ecosystem restoration. Microb Biotechnol. 2023; 16(12):2212-2222. PMC: 10686123. DOI: 10.1111/1751-7915.14322. View

16.

Gamez R, Cardinale M, Montes M, Ramirez S, Schnell S, Rodriguez F . Screening, plant growth promotion and root colonization pattern of two rhizobacteria (Pseudomonas fluorescens Ps006 and Bacillus amyloliquefaciens Bs006) on banana cv. Williams (Musa acuminata Colla). Microbiol Res. 2019; 220:12-20. DOI: 10.1016/j.micres.2018.11.006. View

17.

Zhao S, Garcia D, Zhao Y, Huang D . Hydro-Electro Hybrid Priming Promotes Carrot ( L.) Seed Germination by Activating Lipid Utilization and Respiratory Metabolism. Int J Mol Sci. 2021; 22(20). PMC: 8538415. DOI: 10.3390/ijms222011090. View

18.

Ercan I, Tombuloglu H, Alqahtani N, Alotaibi B, Bamhrez M, Alshumrani R . Magnetic field effects on the magnetic properties, germination, chlorophyll fluorescence, and nutrient content of barley (Hordeum vulgare L.). Plant Physiol Biochem. 2021; 170:36-48. DOI: 10.1016/j.plaphy.2021.11.033. View

19.

Poveda J, Gonzalez-Andres F . Bacillus as a source of phytohormones for use in agriculture. Appl Microbiol Biotechnol. 2021; 105(23):8629-8645. DOI: 10.1007/s00253-021-11492-8. View

20.

Bhardwaj J, Anand A, Nagarajan S . Biochemical and biophysical changes associated with magnetopriming in germinating cucumber seeds. Plant Physiol Biochem. 2012; 57:67-73. DOI: 10.1016/j.plaphy.2012.05.008. View