A New Pro-197-Ile Mutation in Associated with Acetolactate Synthase-Inhibiting Herbicide Resistance

Overview

Journal Plants (Basel)

Date 2025 Feb 26

PMID 40006784

Authors

Meijing Ji

Haiyan Yu

Hailan Cui

Jingchao Chen

Jialin Yu

Xiangju Li

Affiliations

Soon will be listed here.

Abstract

Palmer amaranth ( S. Watson), native to North America, is one of the most prominent invasive weed species on agricultural land. Acetolactate synthase (ALS)-resistant () is widespread, while the research focus on resistance pattern and molecular basis of to imazethapyr is seldom documented in China. An population that survived the recommended rate of imazethapyr was collected in Shandong Province, China. The resistant mechanism and pattern of to imazethapyr was investigated. Dose-response assay showed that the resistant (R) population displayed a high resistance level (292.5-fold) to imazethapyr compared with the susceptible (S) population. Sequence analysis of the gene revealed that nucleotide mutations resulted in three resistance-conferring amino acid substitutions, Pro-197-Ile, Trp-574-Leu, and Ser-653-Asp, in the individual plants of the R population. An in vitro enzyme assay indicated that the ALS was relatively unsusceptible to imazethapyr in the R population, showing a resistance index of 88.6-fold. gene expression and copy number did not confer resistance to imazethapyr in the R population. Pro-197-Ile is the first reported amino acid substitution conferring ALS resistance to . This is the first case of an imazethapyr-resistant biotype in China.

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