Sulfide-Responsive Transcription Control in

Overview

Journal Microorganisms

Specialty Microbiology

Date 2025 Feb 26

PMID 40005711

Authors

Koichi Hori

Rajalakshmi Balasubramanian

Shinji Masuda

Affiliations

Soon will be listed here.

Abstract

To elucidate the mechanism of large-scale transcriptional changes dependent on sulfide in , a large-scale RNA-sequencing analysis was performed on wild-type and sulfide-responsive transcription factor YgaV deletion mutants grown under three conditions: aerobic, semi-aerobic, and semi-aerobic with sulfide. The resulting dataset from these six conditions was subjected to principal component analysis, which categorized the data into five principal components. Estimation of the typical gene expression regulatory mechanisms in each category suggested the presence of mechanisms that are dependent on sulfide but independent of YgaV, as well as those that depend on YgaV but not on sulfide. In YgaV-dependent transcriptional regulation, YgaV was found to function as both a repressor and an activator. These results support the previous hypothesis that YgaV acts as a global regulator responsible for redox homeostasis.

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