Changes in the Expression Profile of Growth-Associated Protein 43 in Degenerative Lumbosacral Stenosis

Overview

Journal J Clin Med

Specialty General Medicine

Date 2025 Feb 26

PMID 40004753

Authors

Dawid Sobanski

Malgorzata Sobanska

Rafal Staszkiewicz

Damian Strojny

Beniamin Oskar Grabarek

Affiliations

Soon will be listed here.

Abstract

Degenerative spinal stenosis is a common condition associated with structural degeneration and pain, yet its molecular underpinnings remain incompletely understood. Growth-associated protein 43 (GAP-43), a key player in neuronal plasticity and regeneration, may serve as a biomarker for disease progression and pain severity. This study investigates the expression of GAP-43 at the mRNA and protein levels in the ligamentum flavum of affected patients. Samples were collected from 96 patients with degenerative spinal stenosis and 85 controls. mRNA expression was analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), while protein levels were quantified via enzyme-linked immunosorbent assay (ELISA) and Western blot. Pain severity was assessed using the visual analog scale (VAS), and associations with lifestyle factors were analyzed. mRNA expression was significantly downregulated in the study group compared to the controls (fold change = 0.58 ± 0.12, < 0.05), with an inverse correlation to VAS pain severity (fold change = 0.76 at VAS 4 vs. 0.36 at VAS 10). Conversely, GAP-43 protein levels were markedly elevated in the study group (5.57 ± 0.21 ng/mL) when compared to controls (0.54 ± 0.87 ng/mL, < 0.0001). Protein levels were also correlated with lifestyle factors, including smoking and alcohol consumption ( < 0.05). GAP-43 shows potential as a biomarker for pain severity and disease progression in degenerative spinal stenosis, in a manner influenced by lifestyle factors. Further research is needed to explore its diagnostic and therapeutic applications.

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