Investigating Polyhydroxyalkanoate Synthesis for Insights into Drug Resistance in Pv.

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2025 Feb 26

PMID 40004067

Authors

Qingbiao Xie

Guangshu Lao

Yukai Fang

Xue Gao

Zheng Tan

Weiguo Miao

Pengfei Jin

Affiliations

Soon will be listed here.

Abstract

Polyhydroxyalkanoates (PHAs), synthesized by to endure adverse conditions, are primarily regulated by the critical genes and . Poly-3-hydroxybutyrate (PHB), a common polyhydroxyalkanoate (PHA), has been implicated in metabolism, pathogenicity, and various physiological processes in pv. (). In this study, we investigated the effects of HN-2 using n-butanol extract (HN-2 n-butanol extract) derived from on . The results showed that HN-2 n-butanol extract could induce PHB accumulation in , potentially via surfactin. Moreover, examination of drug resistance, pathogenicity, and morphological characteristics of revealed PHB played a significant role in the drug resistance, pathogenicity, membrane integrity, and growth rate of strains following the deletion of and . The ∆ strain was the most significant, with a growth rate reduced to 58.19% of the at 36 h and an inhibition zone 57.46% larger than that of by HN-2 n-butanol extract. Transmission electron microscopy further revealed blank spots in after treatment, with the fewest spots observed in ∆, indicating its impaired ability to repair and maintain membrane integrity. These findings offer valuable insights that could serve as a foundation for elucidating the mechanisms of drug resistance and future research on preventing -induced diseases.

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