Genome-Wide Identification and Drought-Responsive Functional Analysis of the Gene Family in Potato ( L.)

Overview

Journal Antioxidants (Basel)

Date 2025 Feb 26

PMID 40002423

Authors

Ningfan Shi

Youfang Fan

Wei Zhang

Zhijia Zhang

Zhuanfang Pu

Zhongrun Li

Lijun Hu

Zhenzhen Bi

Panfeng Yao

Yuhui Liu

Zhen Liu

Jiangping Bai

Chao Sun

Affiliations

Soon will be listed here.

Abstract

Glutathione S-transferases () play crucial roles in crop stress tolerance through protection against oxidative damage. In this study, we conducted genome-wide identification and expression analysis of the gene family in the autotetraploid potato cultivar Cooperative-88 (C88) using bioinformatic approaches. We identified 366 genes in the potato genome, which were classified into 10 subfamilies. Chromosomal mapping revealed that were distributed across all 12 chromosomes, with 13 tandem duplication events observed in three subfamilies. Analysis of protein sequences identified 10 conserved motifs, with motif 1 potentially representing the domain. Analysis of cis-acting elements in the promoter regions suggested their involvement in stress response pathways. RNA-seq analysis revealed that most responded to both drought stress and DNA demethylation treatments. Quantitative PCR validation of 16 selected identified four members that showed strong responses to both treatments, with distinct expression patterns between drought-tolerant (QS9) and drought-sensitive (ATL) varieties. Transient expression assays in tobacco demonstrated that these four enhanced drought tolerance and may be regulated through DNA methylation pathways, though the precise mechanisms require further investigation. These findings provide a theoretical foundation for understanding the response and epigenetic regulation of potato genes under drought stress.

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