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Characterization of Parasite Antigens from Human Hydatid Cyst Fluid by SDS-PAGE and IEF

Overview
Journal Med Microbiol Immunol
Specialty Allergy & Immunology
Date 1985 Jan 1
PMID 4000065
Citations 5
Authors
R DAmelio
O Pontesilli
R Dayal
F De Rosa
M Barnet
A Teggi
G BRIGHOUSE
P H Lambert
Affiliations
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Abstract

Combining high resolution power of SDS-PAGE and IEF with the specific immunological recognition of a human antiserum directed against Echinococcus granulosus antigens, we could identify, in 4 hydatid cystic fluids of human origin, 4 antigens with a molecular weight in the range 32-13 KD, and an antigen of 200 KD which, in reducing conditions, gave 2 bands of 67 and 52 KD. In addition, mainly in one of the cystic fluids, there were at least another 4 specific non-reducible bands with a molecular weight ranging from 80 to 40 KD. Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8.

Citing Articles

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PMID: 10882649 PMC: 95911. DOI: 10.1128/CDLI.7.4.549-552.2000.

Immunological diagnosis of human cystic echinococcosis: utility of discriminant analysis applied to the enzyme-linked immunoelectrotransfer blot.

Gadea I, Ayala G, Diago M, Cunat A, de Lomas J Clin Diagn Lab Immunol. 1999; 6(4):504-8.

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Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens.

Helbig M, Frosch P, Kern P, Frosch M J Clin Microbiol. 1993; 31(12):3211-5.

PMID: 8308113 PMC: 266377. DOI: 10.1128/jcm.31.12.3211-3215.1993.

Spectrotypic analysis of humoral response in human hydatidosis.

DAmelio R, Del Giudice G, De Rosa F, BRIGHOUSE G, Teggi A, Lambert P Clin Exp Immunol. 1989; 76(1):117-20.

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Hydatid disease: analysis of parasite antigens in circulating immune complexes and in preformed hydatid antigen-antibody complexes.

DAmelio R, De Rosa F, Pontesilli O, Dayal R, BRIGHOUSE G, Teggi A Med Microbiol Immunol. 1989; 178(3):177-86.

PMID: 2664438 DOI: 10.1007/BF00198016.

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