Integrative Evidence Reveals a New Species of Hysterothylacium (Nematoda: Ascaridoidea), with the Characterization of Its Complete Mitochondrial Genome

Overview

Journal Int J Parasitol Parasites Wildl

Date 2025 Feb 25

PMID 39995494

Authors

Hui-Xia Chen

Hai-Xia Qiao

Wen-Ting Zhao

Xiao-Hong Gu

Liang Li

Affiliations

Soon will be listed here.

Abstract

The genus (Ascaridida: Raphidascarididae) is among the commonest groups of parasitic nematodes occurring in the digestive tract of marine fishes. In the present study, a new species of , sp. n. collected from (Jordan & Hubbs) and Houttuyn (Perciformes: Uranoscopidae) in the Chinese waters was described using integrative methods, including light and scanning electron microscopy, and ASAP and BI analyses based on the ITS sequence data. The complete mitochondrial genome of the new species was sequenced and annotated, which represents the first mitogenomic data for the genus , and also for the family Raphidascarididae. The mitogenome of sp. n. is 14059 bp in length, including 12 protein coding genes (missing 8), 22 tRNA genes, 2 rRNA genes and 2 non-coding regions, which has 67.0% of overall A + T content, and represents the lowest level of A + T content in the ascaridoid mitogenomes reported so far. Molecular phylogenetic results suggested a close affinity between sp. n. and in the genus .

References

Yamaguti S . Studies on the helminth fauna of Japan. 57. Nematodes of fishes. III. J Helminthol. 1961; Suppl:217-28. DOI: 10.1017/s0022149x00017788. View

Liu S, Liu G, Zhu X, Weng Y . The complete mitochondrial genome of Pseudoterranova azarasi and comparative analysis with other anisakid nematodes. Infect Genet Evol. 2015; 33:293-8. DOI: 10.1016/j.meegid.2015.05.018. View

Li M, Lin R, Song H, Wu X, Zhu X . The complete mitochondrial genomes for three Toxocara species of human and animal health significance. BMC Genomics. 2008; 9:224. PMC: 2396643. DOI: 10.1186/1471-2164-9-224. View

Shamsi S, Ghadam M, Suthar J, Ebrahimzadeh Mousavi H, Soltani M, Mirzargar S . Occurrence of ascaridoid nematodes in selected edible fish from the Persian Gulf and description of Hysterothylacium larval type XV and Hysterothylacium persicum n. sp. (Nematoda: Raphidascarididae). Int J Food Microbiol. 2016; 236:65-73. DOI: 10.1016/j.ijfoodmicro.2016.07.006. View

Guo N, Chen H, Zhang L, Zhang J, Yang L, Li L . Infection and molecular identification of ascaridoid nematodes from the important marine food fish Japanese threadfin bream Nemipterus japonicus (Bloch) (Perciformes: Nemipteridae) in China. Infect Genet Evol. 2020; 85:104562. DOI: 10.1016/j.meegid.2020.104562. View

Cock P, Antao T, Chang J, Chapman B, Cox C, Dalke A . Biopython: freely available Python tools for computational molecular biology and bioinformatics. Bioinformatics. 2009; 25(11):1422-3. PMC: 2682512. DOI: 10.1093/bioinformatics/btp163. View

Park Y, Kim W, Park J . The complete mitochondrial genome of human parasitic roundworm, Ascaris lumbricoides. Mitochondrial DNA. 2011; 22(4):91-3. DOI: 10.3109/19401736.2011.624608. View

Zhao J, Zhao W, Ali A, Chen H, Li L . Morphological variability, ultrastructure and molecular characterisation of Hysterothylacium reliquens (Norris & Overstreet, 1975) (Nematoda: Raphidascarididae) from the oriental sole Brachirus orientalis (Bloch & Schneider) (Pleuronectiformes:.... Parasitol Int. 2016; 66(1):831-838. DOI: 10.1016/j.parint.2016.09.012. View

Reuter J, Mathews D . RNAstructure: software for RNA secondary structure prediction and analysis. BMC Bioinformatics. 2010; 11:129. PMC: 2984261. DOI: 10.1186/1471-2105-11-129. View

10.

Xie Y, Wang L, Chen Y, Wang Z, Zhu P, Hu Z . The Complete Mitogenome of : Novel In-Sights into the Phylogenetics in Toxocaridae. Animals (Basel). 2022; 12(24). PMC: 9774135. DOI: 10.3390/ani12243546. View

11.

Pekmezci G, Yardimci B, Emek Onuk E, Umur S . Molecular characterization of Hysterothylacium fabri (Nematoda: Anisakidae) from Zeus faber (Pisces: Zeidae) caught off the Mediterranean coasts of Turkey based on nuclear ribosomal and mitochondrial DNA sequences. Parasitol Int. 2013; 63(1):127-31. DOI: 10.1016/j.parint.2013.10.006. View

12.

Jin J, Yu W, Yang J, Song Y, dePamphilis C, Yi T . GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes. Genome Biol. 2020; 21(1):241. PMC: 7488116. DOI: 10.1186/s13059-020-02154-5. View

13.

Moravec F, Justine J . Anisakid nematodes (Nematoda: Anisakidae) from the marine fishes Plectropomus laevis Lacépède (Serranidae) and Sphyraena qenie Klunzinger (Sphyraenidae) off New Caledonia, including two new species of Hysterothylacium Ward & Magath, 1917. Syst Parasitol. 2015; 92(3):181-95. DOI: 10.1007/s11230-015-9597-5. View

14.

WOLSTENHOLME D, Okimoto R, Macfarlane J . Nucleotide correlations that suggest tertiary interactions in the TV-replacement loop-containing mitochondrial tRNAs of the nematodes, Caenorhabditis elegans and Ascaris suum. Nucleic Acids Res. 1994; 22(20):4300-6. PMC: 331950. DOI: 10.1093/nar/22.20.4300. View

15.

Costa A, Graci S, Cammilleri G, Buscemi M, Collura R, Vella A . Molecular Identification of Hysterothylacium spp. In Fishes From the Southern Mediterranean Sea (Southern Italy). J Parasitol. 2018; 104(4):398-406. DOI: 10.1645/16-60. View

16.

Gopar-Merino L, Osorio-Sarabia D, Garcia-Prieto L . A new species of Hysterothylacium (Nematoda: Anisakidae) parasite of Ariopsis guatemalensis (Osteichthyes: Ariidae) from Tres Palos lagoon, Mexico. J Parasitol. 2006; 91(4):909-14. DOI: 10.1645/GE-411R.1. View

17.

Li L, Liu Y, Zhang L . Morphological and genetic characterization of Hysterothylacium zhoushanensis sp. nov. (Ascaridida: Anisakidae) from the flatfish Pseudorhombus oligodon (Bleeker) (Pleuronectiformes: Paralichthyidae) in the East China Sea. Parasitol Res. 2012; 111(6):2393-401. DOI: 10.1007/s00436-012-3095-3. View

18.

Zhao J, Tu G, Wu X, Li C . Characterization of the complete mitochondrial genome of Ortleppascaris sinensis (Nematoda: Heterocheilidae) and comparative mitogenomic analysis of eighteen Ascaridida nematodes. J Helminthol. 2017; 92(3):369-378. DOI: 10.1017/S0022149X17000542. View

19.

Knoff M, Felizardo N, Iniguez A, Maldonado Jr A, Torres E, Pinto R . Genetic and morphological characterisation of a new species of the genus Hysterothylacium (Nematoda) from Paralichthys isosceles Jordan, 1890 (Pisces: Teleostei) of the Neotropical Region, state of Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz. 2012; 107(2):186-93. DOI: 10.1590/s0074-02762012000200006. View

20.

Tedesco P, Gustinelli A, Caffara M, Patarnello P, Terlizzi A, Fioravanti M . Hysterothylacium fabri (Nematoda: Raphidascarididae) in Mullus surmuletus (Perciformes: Mullidae) and Uranoscopus scaber (Perciformes: Uranoscopidae) from the Mediterranean. J Parasitol. 2018; 104(3):262-274. DOI: 10.1645/17-115. View