The Role of MATN3 in Cancer Prognosis and Immune Infiltration Across Multiple Tumor Types

Overview

Journal J Cancer

Specialty Oncology

Date 2025 Feb 24

PMID 39991588

Authors

Chongjiu Qin

Haifei Qin

Haixiang Xie

Yuhua Li

Aoyang Bi

Xiwen Liao

Kejian Yang

Chunmiao Lu

Tao Peng

Guangzhi Zhu

Affiliations

Soon will be listed here.

Abstract

MATN3 is a member of the matrix protein family and is involved in the regulation of osteoarthritis as well as the development of gastric cancer. We investigated the role of MATN3 in pan-cancer and validated this result by experiments. We applied multiple databases to explore the expression of MATN3 in 33 types of tumors. Kaplan-Meier survival analysis is performed to understand the effect of MATN3 on Prognostic value in patients with different cancer types. The TIMER database was applied to explore the relationship between MATN3 and immune checkpoint genes, immunomodulatory genes, and immune infiltration, the Sanger box was applied to explore the relationship between MATN3 and methylation, the Genomic Cancer Analysis database was utilized to explore the relationship between MATN3 expression and pharmacological sensitivity, and the STRING database was used to explore the co-expressed genes and to complete the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Data from The Cancer Genome Atlas as well as Genotype-Tissue Expression databases were statistically analyzed and visualized using the R software. Immunohistochemistry and Western blotting for detection of MATN3 expression. CCK-8 and clone formation were used to detect cell proliferation, Wound-healing assay and transwell invasion were used to detect cell migration and invasion ability. MATN3 is overexpressed in most cancer types, indicating a poorer prognosis. It is closely linked to methylation, immunomodulatory genes, and immune checkpoint genes, contributing to immune infiltration in various cancer types. experiments showed that silencing MATN3 inhibited cell proliferation, migration, and invasion ability. MATN3 is involved in the immune infiltration of cancer and affects the prognosis of many cancer types, and can be used as an immune as well as prognostic biomarker for pan-cancer.

References

Liu C, Hu F, Xie G, Miao Y, Li X, Zeng Y . GSCA: an integrated platform for gene set cancer analysis at genomic, pharmacogenomic and immunogenomic levels. Brief Bioinform. 2022; 24(1). DOI: 10.1093/bib/bbac558. View

Li T, Fan J, Wang B, Traugh N, Chen Q, Liu J . TIMER: A Web Server for Comprehensive Analysis of Tumor-Infiltrating Immune Cells. Cancer Res. 2017; 77(21):e108-e110. PMC: 6042652. DOI: 10.1158/0008-5472.CAN-17-0307. View

Wu L, Qu X . Cancer biomarker detection: recent achievements and challenges. Chem Soc Rev. 2015; 44(10):2963-97. DOI: 10.1039/c4cs00370e. View

Li Y, Ge D, Lu C . The SMART App: an interactive web application for comprehensive DNA methylation analysis and visualization. Epigenetics Chromatin. 2019; 12(1):71. PMC: 6894252. DOI: 10.1186/s13072-019-0316-3. View

Jardim D, Goodman A, Gagliato D, Kurzrock R . The Challenges of Tumor Mutational Burden as an Immunotherapy Biomarker. Cancer Cell. 2020; 39(2):154-173. PMC: 7878292. DOI: 10.1016/j.ccell.2020.10.001. View

Franz M, Rodriguez H, Lopes C, Zuberi K, Montojo J, Bader G . GeneMANIA update 2018. Nucleic Acids Res. 2018; 46(W1):W60-W64. PMC: 6030815. DOI: 10.1093/nar/gky311. View

Liu X, Wu H, Liu Z . An Integrative Human Pan-Cancer Analysis of Cyclin-Dependent Kinase 1 (CDK1). Cancers (Basel). 2022; 14(11). PMC: 9179585. DOI: 10.3390/cancers14112658. View

Sun L, Wang H, Xu D, Yu S, Zhang L, Li X . Lapatinib induces mitochondrial dysfunction to enhance oxidative stress and ferroptosis in doxorubicin-induced cardiomyocytes via inhibition of PI3K/AKT signaling pathway. Bioengineered. 2021; 13(1):48-60. PMC: 8805895. DOI: 10.1080/21655979.2021.2004980. View

Zhu J, Gao Y, Wang Y, Zhan Q, Feng H, Luo X . Molecular insights into DNA recognition and methylation by non-canonical type I restriction-modification systems. Nat Commun. 2022; 13(1):6391. PMC: 9613975. DOI: 10.1038/s41467-022-34085-z. View

10.

Li J, Xie B, Wang H, Wang Q, Wu Y . Investigating MATN3 and ASPN as novel drivers of gastric cancer progression via EMT pathways. Hum Mol Genet. 2024; 33(23):2035-2050. DOI: 10.1093/hmg/ddae129. View

11.

Han X, Wang M, Zhao Y, Yang Y, Yang Y . RNA methylations in human cancers. Semin Cancer Biol. 2020; 75:97-115. DOI: 10.1016/j.semcancer.2020.11.007. View

12.

Zhou X, Liang T, Deng J, Ng K, Li M, Lv C . Differential and Prognostic Significance of HOXB7 in Gliomas. Front Cell Dev Biol. 2021; 9:697086. PMC: 8385304. DOI: 10.3389/fcell.2021.697086. View

13.

Qi Y, Liu Z, Hong L, Li P, Ling Z . Methyltransferase-like proteins in cancer biology and potential therapeutic targeting. J Hematol Oncol. 2023; 16(1):89. PMC: 10394802. DOI: 10.1186/s13045-023-01477-7. View

14.

Higgins S, Barletta J . Applications of Immunohistochemistry to Endocrine Pathology. Adv Anat Pathol. 2018; 25(6):413-429. DOI: 10.1097/PAP.0000000000000209. View

15.

Zhang C, Liu X, Jin S, Chen Y, Guo R . Ferroptosis in cancer therapy: a novel approach to reversing drug resistance. Mol Cancer. 2022; 21(1):47. PMC: 8840702. DOI: 10.1186/s12943-022-01530-y. View

16.

Xu C, Jiang Z, Shao L, Zhao Z, Fan X, Sui X . β-Elemene enhances erlotinib sensitivity through induction of ferroptosis by upregulating lncRNA H19 in EGFR-mutant non-small cell lung cancer. Pharmacol Res. 2023; 191:106739. DOI: 10.1016/j.phrs.2023.106739. View

17.

Mandrekar J . Receiver operating characteristic curve in diagnostic test assessment. J Thorac Oncol. 2010; 5(9):1315-6. DOI: 10.1097/JTO.0b013e3181ec173d. View

18.

Pettersson M, Vaz R, Hammarsjo A, Eisfeldt J, Carvalho C, Hofmeister W . Alu-Alu mediated intragenic duplications in IFT81 and MATN3 are associated with skeletal dysplasias. Hum Mutat. 2018; 39(10):1456-1467. DOI: 10.1002/humu.23605. View

19.

Collisson E, Bailey P, Chang D, Biankin A . Molecular subtypes of pancreatic cancer. Nat Rev Gastroenterol Hepatol. 2019; 16(4):207-220. DOI: 10.1038/s41575-019-0109-y. View

20.

Modhukur V, Iljasenko T, Metsalu T, Lokk K, Laisk-Podar T, Vilo J . MethSurv: a web tool to perform multivariable survival analysis using DNA methylation data. Epigenomics. 2017; 10(3):277-288. DOI: 10.2217/epi-2017-0118. View