Gene Expression Changes Precede Elevated Mechanical Sensitivity in the Mouse Intervertebral Disc Injury Model

Overview

Journal JOR Spine

Specialty Orthopedics

Date 2025 Feb 24

PMID 39989623

Authors

Zuozhen Tian

Ken Chen

Frances S Shofer

Brianna Ciesielski

Huan Wang

W Timothy OBrien

Ling Qin

Yejia Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Back pain after intervertebral disc (IVD) injury is a common clinical problem. Previous work examining early molecular changes post injury mainly used a candidate marker approach.

Methods: In this study, gene expression in the injured and intact mouse tail IVDs was determined with a nonbiased whole transcriptome approach and related to subsequent pain behavior. Mouse tail IVD injury was induced by a needle puncture. Whole murine transcriptome was determined by RNASeq. Transcriptomes of injured IVDs were compared with those of intact controls by bioinformatic methods. Mechanical allodynia was assessed by the Von Frey method.

Results: Among the 17,722 murine genes with meaningful expressions, 7242 genes were differentially expressed ( ⟨ 0.01). Ontology study of upregulated genes revealed that leukocyte migration was the most enriched biological process, and network analysis showed that Tnfa had the most protein-protein interactions. The most enriched downregulated pathways were related to the pattern specification process. Mechanical allodynia persisted at the 4-week end point.

Conclusion: The RNASeq data revealed numerous early genes that participate in inflammation and repair processes post IVD injury. Mechanical allodynia followed these gene expression changes.

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