Saccharomyces Cerevisiae Fermentation of High Molecular Weight Hyaluronic Acid Enhanced the Antioxidant Capacity in Skin Fibroblasts

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 2025 Feb 20

PMID 39979606

Authors

Meng Zhang

Doudou Shi

Mimi Cui

Jinyong Li

Wenjing Cheng

Chunhong Pu

Jiachan Zhang

Changtao Wang

Affiliations

Soon will be listed here.

Abstract

In this study, fermented hyaluronic acid (FHA) was obtained by fermentation of hyaluronic acid (HA) with molecular weight ranging from 1200 to 1300 kDa with two strains of Saccharomyces cerevicae (CICC 1308 and CICC 1747). Active substance content and spectral analysis revealed a substantial decrease in FHA molecular weight, accompanied by changes in monosaccharide composition, yet the chemical structure of FHA remained unchanged. Secondly, FHA exhibited a strong ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals. In hydrogen peroxide (HO)-induced human skin fibroblasts (HSF), FHA enhanced superoxide dismutase (SOD) and catalase (CAT) activities and reduced malondialdehyde (MDA) levels, reversing oxidative stress via the Nrf2/Keap1/HO-1 signaling pathway. These findings highlight FHA's potential as a natural cosmetic ingredient. Future research should investigate its long-term effects and potential applications in skin care treatments.

References

Alcantara L, de Sousa J, Andrade F, Teixeira E, Cerqueira M, da Silva A . Extraction and characterization of hyaluronic acid from the eyeball of Nile Tilapia (Oreochromis niloticus). Int J Biol Macromol. 2022; 226:172-183. DOI: 10.1016/j.ijbiomac.2022.12.016. View

Anido J, Fernandez J, Genol I, Ribe N, Perez Sevilla G . Recommendations for the treatment of tear trough deformity with cross-linked hyaluronic acid filler. J Cosmet Dermatol. 2020; 20(1):6-17. PMC: 7818415. DOI: 10.1111/jocd.13475. View

Arevalo-Villena M, Briones-Perez A, Corbo M, Sinigaglia M, Bevilacqua A . Biotechnological application of yeasts in food science: Starter cultures, probiotics and enzyme production. J Appl Microbiol. 2017; 123(6):1360-1372. DOI: 10.1111/jam.13548. View

Chen F, Huang G, Yang Z, Hou Y . Antioxidant activity of Momordica charantia polysaccharide and its derivatives. Int J Biol Macromol. 2019; 138:673-680. DOI: 10.1016/j.ijbiomac.2019.07.129. View

Chen H, Qin J, Hu Y . Efficient Degradation of High-Molecular-Weight Hyaluronic Acid by a Combination of Ultrasound, Hydrogen Peroxide, and Copper Ion. Molecules. 2019; 24(3). PMC: 6384647. DOI: 10.3390/molecules24030617. View

Chien L, Lee C . Enhanced hyaluronic acid production in Bacillus subtilis by coexpressing bacterial hemoglobin. Biotechnol Prog. 2007; 23(5):1017-22. DOI: 10.1021/bp070036w. View

DeAngelis P . Hyaluronan synthases: fascinating glycosyltransferases from vertebrates, bacterial pathogens, and algal viruses. Cell Mol Life Sci. 2001; 56(7-8):670-82. PMC: 11147114. DOI: 10.1007/s000180050461. View

Duan G, Yu X . Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia. Int J Biol Macromol. 2019; 137:1112-1120. DOI: 10.1016/j.ijbiomac.2019.06.177. View

Duarte M, Carvalho M, de Carvalho N, Azevedo-Silva J, Mendes A, Ribeiro I . Skincare potential of a sustainable postbiotic extract produced through sugarcane straw fermentation by Saccharomyces cerevisiae. Biofactors. 2023; 49(5):1038-1060. DOI: 10.1002/biof.1975. View

10.

Fang J, Islam R, Gao S, Zhang C, Kunisaki R, Sakaguchi S . Expression Dynamics of Heme Oxygenase-1 in Tumor Cells and the Host Contributes to the Progression of Tumors. J Pers Med. 2021; 11(12). PMC: 8704574. DOI: 10.3390/jpm11121340. View

11.

Fang Z, Ma M, Wang Y, Dai W, Shang Q, Yu G . Degradation and fermentation of hyaluronic acid by Bacteroides spp. from the human gut microbiota. Carbohydr Polym. 2024; 334:122074. DOI: 10.1016/j.carbpol.2024.122074. View

12.

Kim J, Coradetti S, Kim Y, Gao Y, Yaegashi J, Zucker J . Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in . Front Bioeng Biotechnol. 2021; 8:612832. PMC: 7873862. DOI: 10.3389/fbioe.2020.612832. View

13.

Guo Q, Chen Z, Kumar Santhanam R, Xu L, Gao X, Ma Q . Hypoglycemic effects of polysaccharides from corn silk (Maydis stigma) and their beneficial roles via regulating the PI3K/Akt signaling pathway in L6 skeletal muscle myotubes. Int J Biol Macromol. 2018; 121:981-988. DOI: 10.1016/j.ijbiomac.2018.10.100. View

14.

Hafsa J, Chaouch M, Charfeddine B, Rihouey C, Limem K, Le Cerf D . Effect of ultrasonic degradation of hyaluronic acid extracted from rooster comb on antioxidant and antiglycation activities. Pharm Biol. 2016; 55(1):156-163. PMC: 7011968. DOI: 10.1080/13880209.2016.1232740. View

15.

Huang Y, Huang K, Lew W, Fan K, Chang W, Huang H . Gamma-Irradiation-Prepared Low Molecular Weight Hyaluronic Acid Promotes Skin Wound Healing. Polymers (Basel). 2019; 11(7). PMC: 6680453. DOI: 10.3390/polym11071214. View

16.

Hur S, Lee S, Kim Y, Choi I, Kim G . Effect of fermentation on the antioxidant activity in plant-based foods. Food Chem. 2014; 160:346-56. DOI: 10.1016/j.foodchem.2014.03.112. View

17.

Jiang Z, Lu M, You Q . Discovery and Development of Kelch-like ECH-Associated Protein 1. Nuclear Factor Erythroid 2-Related Factor 2 (KEAP1:NRF2) Protein-Protein Interaction Inhibitors: Achievements, Challenges, and Future Directions. J Med Chem. 2016; 59(24):10837-10858. DOI: 10.1021/acs.jmedchem.6b00586. View

18.

Jin P, Kang Z, Yuan P, Du G, Chen J . Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168. Metab Eng. 2016; 35:21-30. DOI: 10.1016/j.ymben.2016.01.008. View

19.

Ke C, Sun L, Qiao D, Wang D, Zeng X . Antioxidant acitivity of low molecular weight hyaluronic acid. Food Chem Toxicol. 2011; 49(10):2670-5. DOI: 10.1016/j.fct.2011.07.020. View

20.

Park J, Lee S, Park D, Seo Y, Kim S . In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti. J Vis Exp. 2020; (166). DOI: 10.3791/61947. View