Somatic and Stem Cell Bank to Study the Contribution of African Ancestry to Dementia: African IPSC Initiative

Overview

Journal medRxiv

Date 2025 Feb 20

PMID 39974113

Authors

Mahmoud B Maina

Murtala B Isah

Jacob A Marsh

Zaid Muhammad

Larema Babazau

Abdulrahman Alkhamis Idris

Ekaterina Aladyeva

Nadia Miller

Emma Starr

Katherine J Miller

Scott Lee

Miguel Minaya

Selina Wray

Oscar Harari

Baba W Goni

Louise C Serpell

Celeste M Karch

Affiliations

Soon will be listed here.

Abstract

Introduction: Africa, home to 1.4 billion people and the highest genetic diversity globally, harbors unique genetic variants crucial for understanding complex diseases like neurodegenerative disorders. However, African populations remain underrepresented in induced pluripotent stem cell (iPSC) collections, limiting the exploration of population-specific disease mechanisms and therapeutic discoveries.

Methods: To address this gap, we established an open-access African Somatic and Stem Cell Bank.

Results: In this initial phase, we generated 10 rigorously characterized iPSC lines from fibroblasts representing five Nigerian ethnic groups and both sexes. These lines underwent extensive profiling for pluripotency, genetic stability, differentiation potential, and Alzheimer's disease and Parkinson's disease risk variants. CRISPR/Cas9 technology was used to introduce frontotemporal dementia-associated mutations (P301L and R406W).

Discussion: This collection offers a renewable, genetically diverse resource to investigate disease pathogenicity in African populations, facilitating breakthroughs in neurodegenerative research, drug discovery, and regenerative medicine.

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