Serum-derived Exosomal MiR-7977 Combined with MiR-451a As a Potential Biomarker for Pancreatic Ductal Adenocarcinoma

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2025 Feb 19

PMID 39972247

Authors

Jia Chen

Xue Zhang

Guanyi Zhang

Fan Zhu

Weiwei Liu

Affiliations

Soon will be listed here.

Abstract

Objectives: To explore the potential of serum exosomal miRNAs as novel biomarkers for pancreatic ductal adenocarcinoma (PDAC).

Methods: Serum exosomal miRNAs were screened and verified by microarray analysis and quantitative real-time PCR (qRT-PCR) in patients with PDAC and healthy controls. The correlation between the clinical characteristics of PDAC and candidate exosomal miRNAs was analyzed, and the diagnostic performance of the candidate biomarkers was evaluated.

Results: Serum exosomal miR-7977 and miR-451a were significantly upregulated in PDAC patients compared with healthy controls, and the levels of miR-7977 and miR-451a in serum exosomes were closely associated with the clinical stage and metastasis of PDAC patients. The area under curve (AUC) values of serum exosomal miR-7977 and miR-451a for PDAC were 0.825 and 0.804 in the training set and 0.796 and 0.830 in the validation set, respectively. A biomarker panel consisting of these two miRNAs resulted in a diagnostic power with an AUC of 0.901 in the training set and 0.918 in the validation set.

Conclusions: Serum exosomal miR-7977 and miR-451a might be diagnostic biomarkers for PDAC. These two miRNAs, when combined, exhibit optimal diagnostic performance.

References

Redis R, Calin S, Yang Y, You M, Calin G . Cell-to-cell miRNA transfer: from body homeostasis to therapy. Pharmacol Ther. 2012; 136(2):169-74. PMC: 3855335. DOI: 10.1016/j.pharmthera.2012.08.003. View

Welsh J, Goberdhan D, ODriscoll L, Buzas E, Blenkiron C, Bussolati B . Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024; 13(2):e12404. PMC: 10850029. DOI: 10.1002/jev2.12404. View

Kok V, Yu C . Cancer-Derived Exosomes: Their Role in Cancer Biology and Biomarker Development. Int J Nanomedicine. 2020; 15:8019-8036. PMC: 7585279. DOI: 10.2147/IJN.S272378. View

El-Mesallamy H, Hamdy N, Zaghloul A, Sallam A . Serum retinol binding protein-4 and neutrophil gelatinase-associated lipocalin are interrelated in pancreatic cancer patients. Scand J Clin Lab Invest. 2012; 72(8):602-7. DOI: 10.3109/00365513.2012.723135. View

Pereira S, Oldfield L, Ney A, Hart P, Keane M, Pandol S . Early detection of pancreatic cancer. Lancet Gastroenterol Hepatol. 2020; 5(7):698-710. PMC: 7380506. DOI: 10.1016/S2468-1253(19)30416-9. View

Odaka H, Hiemori K, Shimoda A, Akiyoshi K, Tateno H . CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma. BMC Gastroenterol. 2022; 22(1):153. PMC: 8962497. DOI: 10.1186/s12876-022-02228-7. View

Takahasi K, Iinuma H, Wada K, Minezaki S, Kawamura S, Kainuma M . Usefulness of exosome-encapsulated microRNA-451a as a minimally invasive biomarker for prediction of recurrence and prognosis in pancreatic ductal adenocarcinoma. J Hepatobiliary Pancreat Sci. 2017; 25(2):155-161. DOI: 10.1002/jhbp.524. View

Santos Cruz M, Tintelnot J, Gagliani N . Roles of microbiota in pancreatic cancer development and treatment. Gut Microbes. 2024; 16(1):2320280. PMC: 10900280. DOI: 10.1080/19490976.2024.2320280. View

Boutabba A, Missaoui F, Dlala A, Kamoun H, Ben Salem K, Gabsi A . Circulating miR-16-5p, miR-92a-3p and miR-451a are biomarkers of lung cancer in Tunisian patients. BMC Cancer. 2024; 24(1):417. PMC: 10996140. DOI: 10.1186/s12885-024-12181-1. View

10.

Tan Y, Luo X, Lv W, Hu W, Zhao C, Xiong M . Tumor-derived exosomal components: the multifaceted roles and mechanisms in breast cancer metastasis. Cell Death Dis. 2021; 12(6):547. PMC: 8155106. DOI: 10.1038/s41419-021-03825-2. View

11.

Li Z, Li L, Diao Y, Wang J, Ye Y, Hao X . Identification of Urinary Exosomal miRNAs for the Non-Invasive Diagnosis of Prostate Cancer. Cancer Manag Res. 2021; 13:25-35. PMC: 7797303. DOI: 10.2147/CMAR.S272140. View

12.

Halbrook C, Lyssiotis C, Pasca di Magliano M, Maitra A . Pancreatic cancer: Advances and challenges. Cell. 2023; 186(8):1729-1754. PMC: 10182830. DOI: 10.1016/j.cell.2023.02.014. View

13.

Rawla P, Sunkara T, Gaduputi V . Epidemiology of Pancreatic Cancer: Global Trends, Etiology and Risk Factors. World J Oncol. 2019; 10(1):10-27. PMC: 6396775. DOI: 10.14740/wjon1166. View

14.

Zhao Z, Liu W . Pancreatic Cancer: A Review of Risk Factors, Diagnosis, and Treatment. Technol Cancer Res Treat. 2020; 19:1533033820962117. PMC: 7768873. DOI: 10.1177/1533033820962117. View

15.

Bray F, Laversanne M, Sung H, Ferlay J, Siegel R, Soerjomataram I . Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024; 74(3):229-263. DOI: 10.3322/caac.21834. View

16.

Fais S, Logozzi M . The Diagnostic and Prognostic Value of Plasmatic Exosome Count in Cancer Patients and in Patients with Other Pathologies. Int J Mol Sci. 2024; 25(2). PMC: 10816819. DOI: 10.3390/ijms25021049. View

17.

Preethi K, Selvakumar S, Ross K, Jayaraman S, Tusubira D, Sekar D . Liquid biopsy: Exosomal microRNAs as novel diagnostic and prognostic biomarkers in cancer. Mol Cancer. 2022; 21(1):54. PMC: 8848669. DOI: 10.1186/s12943-022-01525-9. View

18.

Zou X, Wei J, Huang Z, Zhou X, Lu Z, Zhu W . Identification of a six-miRNA panel in serum benefiting pancreatic cancer diagnosis. Cancer Med. 2019; 8(6):2810-2822. PMC: 6558458. DOI: 10.1002/cam4.2145. View

19.

Uddin M, Al-Hallak M, Philip P, Mohammad R, Viola N, Wagner K . Exosomal microRNA in Pancreatic Cancer Diagnosis, Prognosis, and Treatment: From Bench to Bedside. Cancers (Basel). 2021; 13(11). PMC: 8199777. DOI: 10.3390/cancers13112777. View

20.

Hamdy N, Zaki M, Abdelmaksoud N, Ismail R, Abd-Elmawla M, Rizk N . Insights into the genetic and epigenetic mechanisms governing X-chromosome-linked-miRNAs expression in cancer; a step-toward ncRNA precision. Int J Biol Macromol. 2024; 289:138773. DOI: 10.1016/j.ijbiomac.2024.138773. View