Effects of Lupeol on Experimental Testicular Ischemiareperfusion Damage in Rats

Overview

Journal Ulus Travma Acil Cerrahi Derg

Specialties Emergency Medicine
General Surgery

Date 2025 Feb 18

PMID 39963909

Authors

Abdurrahman Azzam

Ramazan Karabulut

Cem Kaya

Sibel Eryilmaz

Alparslan Kapisiz

Zafer Turkyilmaz

Mehmet Arda Inan

Gizem Yaz Aydin

Ali Atan

Kaan Sonmez

Affiliations

Soon will be listed here.

Abstract

Background: Infertility and organ loss are possible outcomes of testicular torsion, a urological emergency. This study aimed to demonstrate the impact of lupeol on testicular ischemia/reperfusion damage.

Methods: Thirty adult male Sprague-Dawley rats were randomly assigned to five groups: Control (C), Lupeol (L), Ischemia (Isc),Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles following the induction of 720-degree testicular torsion for two hours. In the T1 and T2 groups, 100 mg/kg of lupeol was administered intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour, blood and testicular tissue samples were collected from each rat. Measurements included serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 levels. Histopathological analysis was performed to assess the Johnsen Tubular Biopsy Score (JTBS).

Results: Caspase-3 (2,74+-0,32), MDA (1,71+-0,26), IL-6 (4,92+-0,57), and TNF-ɑ (113,18+-29,77) values increased in Group Isc compared to Group C and significantly decreased in T2 (2+-0,67, 1,16+-0,36, 3,95+-0,17, and 106,13+-12,49) and particularly T1 groups (1,65+-0,50, 0,95+-0,143, 80+-0,35, and 104,86+-8,42) (p=0.001). However, TNF-α levels decreased in both treatment groups, with no statistically significant difference (p=0.768). GSH levels decreased in Group Isc (140,63+-25,71) but increased in T2 (211,58+-95,05) (p=0.753) and particularly in T1 groups (219,9+-48,21) (p=0.078). JTBS was lowest in Group Isc (7,67+-0,25). Improvement was observed in both treatment groups (8,93+-0,16 and 8,82+-0,22) (p=0.001).

Conclusion: This study, which is the first to use lupeol in an experimental testicular torsion model, demonstrated its antioxidant, anti-inflammatory, antiapoptotic, and histopathological damage-reducing and protective effects.

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