Comparative Metabolomics Reveals Streptophenazines with Anti-methicillin-resistant Staphylococcus Aureus Activity Derived from Streptomyces Albovinaceus Strain WA10-1-8 Isolated from Periplaneta Americana

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2025 Feb 18

PMID 39962376

Authors

Liuchong Zhu

Dan Huang

Jinli Tan

Jiaxuan Huang

Ruyu Zhang

Jingyang Liao

Jie Wang

Xiaobao Jin

Affiliations

Soon will be listed here.

Abstract

Background: Streptophenazines, a class of phenazine compounds with a variety of alkyl side chains and activity against methicillin-resistant Staphylococcus aureus (MRSA), are mainly derived from soil or marine microbial secondary metabolites. However, the discovered phenazine compounds still do not meet the needs of the development of anti-MRSA lead compounds. Here, we examined secondary metabolites of Streptomyces albovinaceus WA10-1-8 isolated from Periplaneta americana, for streptophenazines with anti-MRSA activity.

Results: In this study, a guidance method combining high-performance liquid chromatography-ultraviolet (HPLC-UV) with molecular networking analysis was used to isolate and identify a series of streptophenazines (A-T) from S. albovinaceus WA10-1-8. Among them, a new streptophenazine containing a dihydroxyalkyl chain structure named streptophenazine T was isolated and identified for the first time. The results of bioactivity assays showed that streptophenazine T had anti-MRSA activity with a minimum inhibitory concentration (MIC) of 150.23 µM, while the MICs of streptophenazine A, B, G, and F were 37.74-146.12 µM.

Conclusions: This study was the first to report multiple streptophenazine compounds with anti-MRSA activity expressed by Streptomyces isolated from insect niches. These results provided a valuable reference for future exploration of new streptophenazine compounds with activity against drug-resistant bacteria.

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