Quantification of DNA, in Dried Blood, Saliva Tinged with Blood in Comparision with Fresh Blood for Forensic Identification- A Pilot Study

Overview

Journal J Oral Maxillofac Pathol

Date 2025 Feb 14

PMID 39949698

Authors

Kangan Manjunathan

Radhika Manoj Bavle

Mrinalini Menon

Reshma Venugopal

Soumya Makarla

Sankha Nair

Affiliations

Soon will be listed here.

Abstract

Aims And Objectives: To determine if blood-tinged saliva or dried blood yields a good amount of DNA. To compare it with fresh blood samples for authentication.

Materials And Methodology: The study groups: Group A - = 20 normal blood samples, Group B - = 20 of saliva-tinged blood samples and Group C - Dried Blood samples from the same subjects. DNA was isolated from these 60 samples using Xplorogen DNA isolation and extraction kit. The quality and quantity of DNA isolation were determined using a spectrophotometric technique using a Biospectrometer.

Results: The extracted DNA was quantified and the yield was 83.3 ± 9.4, 55.1 ± 7.4, and 73.4 ± 8.7 μg/ml in fresh blood, dried blood, and blood-tinged saliva, respectively. Out of the 60 samples assessed, all 100% samples yielded a sufficiently good DNA content. Extraction from Blood tinged with saliva showed promising results when compared to dried blood and the levels obtained were closely comparable to whole blood (fresh).

Conclusion: Blood is a standard sample for ample DNA yields in forensic studies. We conclude in this study, that blood-tinged saliva gives an equally good sample for utilization in DNA detection in those situations where such samples are traced (even at a later date) at the scene of crime, or mass disasters for forensic analysis.

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