Structural Studies of Human Basement-membrane Collagen with the Use of a Monoclonal Antibody

Overview

Journal Biochem J

Specialty Biochemistry

Date 1985 Apr 1

PMID 3994683

Citations 3

Authors

H Dieringer

D W Hollister

R W Glanville

L Y Sakai

K Kuhn

Affiliations

Soon will be listed here.

Abstract

A monoclonal antibody monospecific for human type IV collagen was used as a structural probe to examine aspects of the macromolecular organization of basement-membrane collagen. Electron-microscopic observation of rotary-shadowed antigen-antibody complexes demonstrated a unique binding site for the antibody 55 +/- 6 nm distant from the 7S cross-linking region of tetrameric type IV collagen. This observation allowed a series of studies that showed: (1) the localization of an intramolecular disulphide bridge within the helical domain of the molecule, (2) the alignment of major peptic-digest fragments of the alpha 1 (IV) chain, and (3) confirmation of the postulated antiparallel arrangement of individual molecules within type IV collagen tetramers.

Citing Articles

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Type VII collagen is a major structural component of anchoring fibrils.

Sakai L, Keene D, Morris N, Burgeson R J Cell Biol. 1986; 103(4):1577-86.

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Characterization of a type IV collagen major cell binding site with affinity to the alpha 1 beta 1 and the alpha 2 beta 1 integrins.

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PMID: 1646206 PMC: 2289033. DOI: 10.1083/jcb.113.6.1475.

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