Proteomic Insights into Fruit-pathogen Interactions: Managing Biotic Stress in Fruit

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2025 Feb 13

PMID 39945834

Authors

Aidhya Irhash Putra

Muhammad Naveed Khan

Nurhaida Kamaruddin

Raja Farhana R Khairuddin

Jameel R Al-Obaidi

Brenda Juana Flores

Luis Fernando Flores

Affiliations

Soon will be listed here.

Abstract

Proteomics has revealed complex immune responses in fruits, leading to the identification of potential disease biomarkers and resistance mechanisms. Fruit diseases caused by fungal and bacterial pathogens present critical challenges to global food security by reducing fruit shelf life and quality. This review explores the molecular dynamics of fruit-pathogen interactions using advanced proteomic techniques. These approaches include mass spectrometry-based identification, gel-based, and gel-free strategies, tailored to the unique compositions of fruit tissues for accurate protein extraction and identification. Proteomic studies reveal pathogen-induced changes in fruit proteomes, including the upregulation of defence-related proteins and suppression of metabolic pathways crucial for pathogen survival. Case studies on tomatoes, apples, and bananas highlight specific pathogen-responsive proteins, such as PR proteins and enzymes involved in ROS scavenging, which play roles in disease resistance mechanisms. The review further demonstrates the utility of proteomic data in identifying early disease biomarkers, guiding genetic improvements for disease resistance, and optimizing pathogen control measures. Integrating proteomic insights with transcriptomics and metabolomics provides a multidimensional understanding of fruit-pathogen interactions, paving the way for innovative solutions in agriculture. Future research should prioritize multi-omics approaches and field-level validations to translate laboratory findings into practical applications. The advancements discussed underscore the transformative role of proteomics in improving food security and sustainability amid challenges posed by climate change and increasing global food demand.

References

Afzaal M, Saeed F, Ateeq H, Akhtar M, Imran A, Ahmed A . Probiotics encapsulated gastroprotective cross-linked microgels: Enhanced viability under stressed conditions with dried apple carrier. Food Sci Nutr. 2023; 11(2):817-827. PMC: 9922151. DOI: 10.1002/fsn3.3116. View

Al-Obaidi J, Saidi N, Ahmad Usuldin S, Hussin S, Yusoff N, Idris A . Comparison of Different Protein Extraction Methods for Gel-Based Proteomic Analysis of Ganoderma spp. Protein J. 2016; 35(2):100-6. DOI: 10.1007/s10930-016-9656-z. View

Al-Obaidi J, Jamaludin A, Abdul Rahman N, Ahmad-Kamil E . How plants respond to heavy metal contamination: a narrative review of proteomic studies and phytoremediation applications. Planta. 2024; 259(5):103. DOI: 10.1007/s00425-024-04378-2. View

Abed Z, Arianejad M, Azizi Z . Mesenchymal stem cell-derived exosomes decrease Hyperplasia in Psoriasis by inducing transforming growth factor β2 (TGF-β2). Mol Biol Rep. 2024; 51(1):635. DOI: 10.1007/s11033-024-09337-4. View

Blanco-Ulate B, Morales-Cruz A, Amrine K, Labavitch J, Powell A, Cantu D . Genome-wide transcriptional profiling of Botrytis cinerea genes targeting plant cell walls during infections of different hosts. Front Plant Sci. 2014; 5:435. PMC: 4153048. DOI: 10.3389/fpls.2014.00435. View

Brajkovic S, Rugen N, Agius C, Berner N, Eckert S, Sakhteman A . Getting Ready for Large-Scale Proteomics in Crop Plants. Nutrients. 2023; 15(3). PMC: 9921824. DOI: 10.3390/nu15030783. View

Bukhat S, Imran A, Javaid S, Shahid M, Majeed A, Naqqash T . Communication of plants with microbial world: Exploring the regulatory networks for PGPR mediated defense signaling. Microbiol Res. 2020; 238:126486. DOI: 10.1016/j.micres.2020.126486. View

Carstens C, Salazar J, Darkoh C . Multistate Outbreaks of Foodborne Illness in the United States Associated With Fresh Produce From 2010 to 2017. Front Microbiol. 2019; 10:2667. PMC: 6883221. DOI: 10.3389/fmicb.2019.02667. View

Wang L, Liu X, Wang X, Pan Z, Geng X, Chen B . Identification and characterization analysis of sulfotransferases (SOTs) gene family in cotton (Gossypium) and its involvement in fiber development. BMC Plant Biol. 2020; 19(1):595. PMC: 6938023. DOI: 10.1186/s12870-019-2190-3. View

10.

Cheung N, Tian L, Liu X, Li X . The Destructive Fungal Pathogen -Insights from Genes Studied with Mutant Analysis. Pathogens. 2020; 9(11). PMC: 7695001. DOI: 10.3390/pathogens9110923. View

11.

Chin C, Teoh E, Chee M, Al-Obaidi J, Rahmad N, Lawson T . Comparative Proteomic Analysis on Fruit Ripening Processes in Two Varieties of Tropical Mango (Mangifera indica). Protein J. 2019; 38(6):704-715. DOI: 10.1007/s10930-019-09868-x. View

12.

DAmbrosio C, Arena S, Rocco M, Verrillo F, Novi G, Viscosi V . Proteomic analysis of apricot fruit during ripening. J Proteomics. 2012; 78:39-57. DOI: 10.1016/j.jprot.2012.11.008. View

13.

De Miccolis Angelini R, Abate D, Rotolo C, Gerin D, Pollastro S, Faretra F . De novo assembly and comparative transcriptome analysis of Monilinia fructicola, Monilinia laxa and Monilinia fructigena, the causal agents of brown rot on stone fruits. BMC Genomics. 2018; 19(1):436. PMC: 5987419. DOI: 10.1186/s12864-018-4817-4. View

14.

Elmore J, Griffin B, Walley J . Advances in functional proteomics to study plant-pathogen interactions. Curr Opin Plant Biol. 2021; 63:102061. DOI: 10.1016/j.pbi.2021.102061. View

15.

Zhang B, Sun S, Luo W, Li J, Fang Q, Zhang D . A new brood-pollination mutualism between Stellera chamaejasme and flower thrips Frankliniella intonsa. BMC Plant Biol. 2021; 21(1):562. PMC: 8628443. DOI: 10.1186/s12870-021-03319-5. View

17.

Gonzalez-Fernandez R, Prats E, Jorrin-Novo J . Proteomics of plant pathogenic fungi. J Biomed Biotechnol. 2010; 2010:932527. PMC: 2878683. DOI: 10.1155/2010/932527. View

18.

Gonzalez-Fernandez R, Jorrin-Novo J . Contribution of proteomics to the study of plant pathogenic fungi. J Proteome Res. 2011; 11(1):3-16. DOI: 10.1021/pr200873p. View

19.

Hussain H, Mustafa Kamal M, Al-Obaidi J, Hamdin N, Ngaini Z, Mohd-Yusuf Y . Proteomics of Sago Palm Towards Identifying Contributory Proteins in Stress-Tolerant Cultivar. Protein J. 2019; 39(1):62-72. DOI: 10.1007/s10930-019-09878-9. View

20.

Kapoor L, Simkin A, George Priya Doss C, Siva R . Fruit ripening: dynamics and integrated analysis of carotenoids and anthocyanins. BMC Plant Biol. 2022; 22(1):27. PMC: 8750800. DOI: 10.1186/s12870-021-03411-w. View

21.

Kilambi H, Manda K, Sanivarapu H, Maurya V, Sharma R, Sreelakshmi Y . Shotgun Proteomics of Tomato Fruits: Evaluation, Optimization and Validation of Sample Preparation Methods and Mass Spectrometric Parameters. Front Plant Sci. 2016; 7:969. PMC: 4925719. DOI: 10.3389/fpls.2016.00969. View