Multidrug-resistant ESBL in Urban Surface Waters and Public Health Implications: A Case Study from Goranchatbari, Dhaka

Overview

Journal Heliyon

Specialty Social Sciences

Date 2025 Feb 13

PMID 39944332

Authors

Md Sakib Hossain

Ahmed Ishtiaque Amin Chowdhury

Mohammad Rafiqul Islam

Ripan Kirtunia

Md Foysal Abedin

Mohammad Atique Ul Alam

Shampa

Sonia Binte Murshed

Md Shadman Sakib

Siam Alam

M Shahjahan Mondal

Zahid Hayat Mahmud

Affiliations

Soon will be listed here.

Abstract

Surface water pollution from rapid urbanization, industrialization, inadequate sanitation, and excessive agrochemical use is a global crisis. In developing countries, water quality is one of the most urgent environmental issues. The presence of pathogenic extended spectrum β-lactamase (ESBL) producing in surface water poses a critical public health concern by increasing the risk of waterborne diseases and spreading multidrug-resistant (MDR) infections. This study addresses the severity of surface water pollution in urban Bangladesh, focusing on the molecular characterization of ESBL in surface waters from the Goranchatbari sub-catchment of Dhaka. Isolates of ESBL underwent analysis for the major ESBL and pathogenic genes, antibiotic susceptibility, biofilm formation, and genetic diversity. However, the existence of was confirmed in every sample. Among 266 isolates, 62 (23.31 %) were phenotypically positive for ESBL, with 58 (93.55 %) carrying at least one of the four ESBL genes: CTX-M, TEM, SHV, and OXA. CTX-M was the most prevalent, found in 55 (88.71 %) isolates. Regarding pathogenicity, 25 (40.32 %) isolates were enteric pathogens, including 24 ETEC and a single EIEC. Four non-diarrheagenic isolates were extraintestinal pathogenic (ExPEC), capable of causing diseases beyond enteric infections. All ESBL isolates were MDR, with high resistance to β-lactam antibiotics, and capable of forming biofilms at 25 °C and 37 °C. ERIC-PCR analysis grouped the isolates into 14 distinct clusters at a 75 % similarity matrix. These water sources critically threaten public health by contaminating nearby freshwater sources with ESBL-producing pathogenic isolates, leading to various hard-to-treat waterborne diseases and risking aquatic biota by deteriorating water quality. Immediate public awareness, proper water treatment, and precise environmental management are needed before using this water for any purpose.

References

Amin M, Saha S, Islam M, Haider S, Hossain M, Chowdhury A . High prevalence of plasmid-mediated quinolone resistance (PMQR) among E. coli from aquatic environments in Bangladesh. PLoS One. 2021; 16(12):e0261970. PMC: 8716050. DOI: 10.1371/journal.pone.0261970. View

Rabbani M, Howlader M, Kabir Y . Detection of multidrug resistant (MDR) bacteria in untreated waste water disposals of hospitals in Dhaka City, Bangladesh. J Glob Antimicrob Resist. 2017; 10:120-125. DOI: 10.1016/j.jgar.2017.04.009. View

Heras J, Dominguez C, Mata E, Pascual V, Lozano C, Torres C . GelJ--a tool for analyzing DNA fingerprint gel images. BMC Bioinformatics. 2015; 16:270. PMC: 4549892. DOI: 10.1186/s12859-015-0703-0. View

Islam M, Rahman A, Hassan J, Rahman M . Extended-spectrum beta-lactamase in isolated from humans, animals, and environments in Bangladesh: A One Health perspective systematic review and meta-analysis. One Health. 2023; 16:100526. PMC: 10288049. DOI: 10.1016/j.onehlt.2023.100526. View

Zurfluh K, Hachler H, Nuesch-Inderbinen M, Stephan R . Characteristics of extended-spectrum β-lactamase- and carbapenemase-producing Enterobacteriaceae Isolates from rivers and lakes in Switzerland. Appl Environ Microbiol. 2013; 79(9):3021-6. PMC: 3623138. DOI: 10.1128/AEM.00054-13. View

Novo A, Andre S, Viana P, Nunes O, Manaia C . Antibiotic resistance, antimicrobial residues and bacterial community composition in urban wastewater. Water Res. 2013; 47(5):1875-87. DOI: 10.1016/j.watres.2013.01.010. View

Jafari A, Aslani M, Bouzari S . Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran. Iran J Microbiol. 2012; 4(3):102-17. PMC: 3465535. View

Annunziato G . Strategies to Overcome Antimicrobial Resistance (AMR) Making Use of Non-Essential Target Inhibitors: A Review. Int J Mol Sci. 2019; 20(23). PMC: 6928725. DOI: 10.3390/ijms20235844. View

Qadri F, Svennerholm A, Faruque A, Sack R . Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev. 2005; 18(3):465-83. PMC: 1195967. DOI: 10.1128/CMR.18.3.465-483.2005. View

10.

Sidhu J, Ahmed W, Hodgers L, Toze S . Occurrence of virulence genes associated with Diarrheagenic pathotypes in Escherichia coli isolates from surface water. Appl Environ Microbiol. 2012; 79(1):328-35. PMC: 3536071. DOI: 10.1128/AEM.02888-12. View

11.

Canton R, Coque T . The CTX-M beta-lactamase pandemic. Curr Opin Microbiol. 2006; 9(5):466-75. DOI: 10.1016/j.mib.2006.08.011. View

12.

Anastasi E, Matthews B, Stratton H, Katouli M . Pathogenic Escherichia coli found in sewage treatment plants and environmental waters. Appl Environ Microbiol. 2012; 78(16):5536-41. PMC: 3406122. DOI: 10.1128/AEM.00657-12. View

13.

Navab-Daneshmand T, Friedrich M, Gachter M, Montealegre M, Mlambo L, Nhiwatiwa T . Contamination across Multiple Environmental Compartments (Soil, Hands, Drinking Water, and Handwashing Water) in Urban Harare: Correlations and Risk Factors. Am J Trop Med Hyg. 2018; 98(3):803-813. PMC: 5930891. DOI: 10.4269/ajtmh.17-0521. View

14.

Seiler C, Berendonk T . Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture. Front Microbiol. 2012; 3:399. PMC: 3522115. DOI: 10.3389/fmicb.2012.00399. View

15.

Kraupner N, Ebmeyer S, Bengtsson-Palme J, Fick J, Kristiansson E, Flach C . Selective concentration for ciprofloxacin resistance in Escherichia coli grown in complex aquatic bacterial biofilms. Environ Int. 2018; 116:255-268. DOI: 10.1016/j.envint.2018.04.029. View

16.

Mahmud Z, Kabir M, Ali S, Moniruzzaman M, Imran K, Nafiz T . Extended-Spectrum Beta-Lactamase-Producing in Drinking Water Samples From a Forcibly Displaced, Densely Populated Community Setting in Bangladesh. Front Public Health. 2020; 8:228. PMC: 7314906. DOI: 10.3389/fpubh.2020.00228. View

17.

Flores-Mireles A, Walker J, Caparon M, Hultgren S . Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol. 2015; 13(5):269-84. PMC: 4457377. DOI: 10.1038/nrmicro3432. View

18.

Chattopadhyay I, J R, Usman T, Varjani S . Exploring the role of microbial biofilm for industrial effluents treatment. Bioengineered. 2022; 13(3):6420-6440. PMC: 8974063. DOI: 10.1080/21655979.2022.2044250. View

19.

Wilkinson J, Boxall A, Kolpin D, Leung K, Lai R, Galban-Malagon C . Pharmaceutical pollution of the world's rivers. Proc Natl Acad Sci U S A. 2022; 119(8). PMC: 8872717. DOI: 10.1073/pnas.2113947119. View

20.

Rehman M, Rashid N, Ashfaq M, Saif A, Ahmad N, Han J . Global risk of pharmaceutical contamination from highly populated developing countries. Chemosphere. 2013; 138:1045-55. DOI: 10.1016/j.chemosphere.2013.02.036. View