Investigation of the Influence of the Extraction System and Seasonality on the Pharmacological Potential of Leaves

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2025 Feb 13

PMID 39942817

Authors

Kidney O G Neves

Samuel O Silva

Marinildo S Cruz

Josiana Moreira Mar

Jaqueline A Bezerra

Edgar A Sanches

Natasha Marques Cassani

Giovanna A Antoniucci

Ana Carolina Gomes Jardim

Francisco C M Chaves

Leonard D R Acho

Emersom S Lima

Marcos B Machado

Alan D C Santos

Affiliations

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Abstract

The chemical complexity of natural products, such as (Kunth) DC. plant, presents a challenge when extracting and identifying bioactive compounds. This study investigates the impact of different extraction systems and seasonal variations on the chemical profile and pharmacological potential of leaves using NMR spectroscopy for chemical analysis and canonical correlation analysis (CCA) for bioactivity correlation. Extracts obtained with methanol (M), ethanol (E), methanol/ethanol (1:1, ME), and methanol/ethanol/water (3:1:1, MEW) were analyzed for antioxidant, antiglycation, and antiviral activities. Quantitative ¹H NMR, combined with the PULCON method, was used to quantify phenolic compounds such as quercetin, myricetin, catechin, and gallic acid. The results showed that the MEW extract obtained in the rainy season exhibited the highest antioxidant and antiglycation activities, with a greater than 93% of advanced-glycation end-products (AGEs) inhibition capacity. Furthermore, our results showed that all the extracts were able to inhibit over 94% of the Zika virus (ZIKV) infection in Vero E6 cells. The CCA established strong correlations between the phenolic compounds and bioactivities, identifying gallic acid, catechin, quercetin, and myricetin as key chemical markers. This study demonstrates the importance of selecting appropriate extraction systems and considering seasonality to optimize the pharmacological potential of leaves and highlights the efficacy of NMR in linking chemical composition with bioactivities.

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