Effects of Aqueous Extract of Waltheria Indica (Sterculiaceae) Leafy Stems on Acetic Acid-induced Ulcerative Colitis in Rats

Overview

Journal Inflammopharmacology

Specialty Pharmacology

Date 2025 Feb 11

PMID 39934536

Authors

Michel Archange Fokam Tagne

Paul Aime Noubissi

Angele Foyet Fondjo

Laurelle Nono Njomguep

Joseph Ngakou Mukam

Selestin Sokeng Dongmo

Rene Kamgang

Affiliations

Soon will be listed here.

Abstract

Ulcerative colitis is one of the inflammatory bowel diseases that manifest itself by uncontrolled inflammation of colon. The objective of this work was to evaluate the effects of aqueous extract of Waltheria indica on acetic acid-induced ulcerative colitis in rats. Six (6) groups of five (5) rats each, were anesthetized with a ketamine (50 mg/kg)/valium (10 mg/kg) mixture after eighteen (18) fasting hours. Colitis was induced by intrarectal administration of 1 mL of acetic acid (5%) in animals. Five (5) hours later, the normal control (NC) and the colitis control (CC) received distilled water (10 mL/kg bw), the positive control (Pre5) received prednisolone (5 mg/kg) and the other three test groups received the W. indica extract at 50 (Wi50), 100 (Wi100) and 200 (Wi200) mg/kg bw, orally for 7 days. At the end of the treatment, the animals were sacrificed and the blood was collected from the carotid artery, part in the ethylenediaminetetraacetate (EDTA) tube for hematological analyzes and part in dry tubes for biochemical assays. The abdomen was then opened, the colon, liver, spleen, lungs and heart were removed, drained, weighed and the indexes of each organ were determined. The extract at 200 mg/kg reduced myeloperoxidase (MPO) and inhibited the production of interleukin-1 beta (IL-1β) and interleukin-6(IL-6) in the colon and serum. The extract significantly increased the blood platelet level of the colitis rats. Thus, these results suggest that Walthera indica extract may have therapeutic potential for the treatment of inflammatory bowel diseases.

References

Baker D, Marta M, Pryce G, Giovannoni G, Schmierer K . Memory B Cells are Major Targets for Effective Immunotherapy in Relapsing Multiple Sclerosis. EBioMedicine. 2017; 16:41-50. PMC: 5474520. DOI: 10.1016/j.ebiom.2017.01.042. View

Barbic M, Willer E, Rothenhofer M, Heilmann J, Furst R, Jurgenliemk G . Spirostanol saponins and esculin from Rusci rhizoma reduce the thrombin-induced hyperpermeability of endothelial cells. Phytochemistry. 2013; 90:106-13. DOI: 10.1016/j.phytochem.2013.02.004. View

Cagin Y, Parlakpinar H, Vardi N, Polat A, Atayan Y, Erdogan M . Effects of dexpanthenol on acetic acid-induced colitis in rats. Exp Ther Med. 2016; 12(5):2958-2964. PMC: 5103730. DOI: 10.3892/etm.2016.3728. View

Cardona F, Andres-Lacueva C, Tulipani S, Tinahones F, Queipo-Ortuno M . Benefits of polyphenols on gut microbiota and implications in human health. J Nutr Biochem. 2013; 24(8):1415-22. DOI: 10.1016/j.jnutbio.2013.05.001. View

Costa C, Incio J, Soares R . Angiogenesis and chronic inflammation: cause or consequence?. Angiogenesis. 2007; 10(3):149-66. DOI: 10.1007/s10456-007-9074-0. View

Fokam Tagne M, Tchoffo A, Noubissi P, Mazo A, Kom B, Ngakou Mukam J . Effects of hydro-ethanolic extract of leaves of Maesa lanceolata (Mursinaceae) on acetic acid-induced ulcerative colitis in rats. Inflammopharmacology. 2021; 29(4):1211-1223. DOI: 10.1007/s10787-021-00825-8. View

Fokam Tagne M, Kom B, Foyet Fondjo A, Noubissi P, Talom Tangue B, Gaffo E . Evaluation of Anticolitis and Antioxidant Properties of (Bixaceae) Leaf Hydroethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats. Curr Ther Res Clin Exp. 2022; 97:100685. PMC: 9539784. DOI: 10.1016/j.curtheres.2022.100685. View

Gremy O, Benderitter M, Linard C . Caffeic acid phenethyl ester modifies the Th1/Th2 balance in ileal mucosa after gamma-irradiation in the rat by modulating the cytokine pattern. World J Gastroenterol. 2006; 12(31):4996-5004. PMC: 4087402. DOI: 10.3748/wjg.v12.i31.4996. View

Heil M, Baumann B, Andary C, Linsenmair K, McKey D . Extraction and quantification of "condensed tannins" as a measure of plant anti-herbivore defence? Revisiting an old problem. Naturwissenschaften. 2002; 89(11):519-24. DOI: 10.1007/s00114-002-0366-3. View

10.

Jagtap A, Shirke S, Phadke A . Effect of polyherbal formulation on experimental models of inflammatory bowel diseases. J Ethnopharmacol. 2004; 90(2-3):195-204. DOI: 10.1016/j.jep.2003.09.042. View

11.

Jurjus A, Khoury N, Reimund J . Animal models of inflammatory bowel disease. J Pharmacol Toxicol Methods. 2004; 50(2):81-92. DOI: 10.1016/j.vascn.2003.12.002. View

12.

Khor B, Gardet A, Xavier R . Genetics and pathogenesis of inflammatory bowel disease. Nature. 2011; 474(7351):307-17. PMC: 3204665. DOI: 10.1038/nature10209. View

13.

Kim H, Oh M, Park K, Heo J, Lee M . Anti-inflammatory activity of sulfate-containing phenolic compounds isolated from the leaves of Myrica rubra. Fitoterapia. 2013; 92:188-93. DOI: 10.1016/j.fitote.2013.10.007. View

14.

Klebanoff S . Myeloperoxidase: friend and foe. J Leukoc Biol. 2005; 77(5):598-625. DOI: 10.1189/jlb.1204697. View

15.

Laczko R, Chang A, Watanabe L, Petelo M, Kahaleua K, Bingham J . Anti-inflammatory activities of Waltheria indica extracts by modulating expression of IL-1B, TNF-α, TNFRII and NF-κB in human macrophages. Inflammopharmacology. 2019; 28(2):525-540. DOI: 10.1007/s10787-019-00658-6. View

16.

Lenoir L, Joubert-Zakeyh J, Texier O, Lamaison J, Vasson M, Felgines C . Aloysia triphylla infusion protects rats against dextran sulfate sodium-induced colonic damage. J Sci Food Agric. 2011; 92(7):1570-2. DOI: 10.1002/jsfa.5544. View

17.

Lungaro L, Costanzini A, Manza F, Barbalinardo M, Gentili D, Guarino M . Impact of Female Gender in Inflammatory Bowel Diseases: A Narrative Review. J Pers Med. 2023; 13(2). PMC: 9958616. DOI: 10.3390/jpm13020165. View

18.

Martinez-Dominguez E, de la Puerta R, Ruiz-Gutierrez V . Protective effects upon experimental inflammation models of a polyphenol-supplemented virgin olive oil diet. Inflamm Res. 2001; 50(2):102-6. DOI: 10.1007/s000110050731. View

19.

Mascaraque C, Gonzalez R, Suarez M, Zarzuelo A, Sanchez de Medina F, Martinez-Augustin O . Intestinal anti-inflammatory activity of apigenin K in two rat colitis models induced by trinitrobenzenesulfonic acid and dextran sulphate sodium. Br J Nutr. 2015; 113(4):618-26. DOI: 10.1017/S0007114514004292. View

20.

Monteillier A, Cretton S, Ciclet O, Marcourt L, Ebrahimi S, Christen P . Cancer chemopreventive activity of compounds isolated from Waltheria indica. J Ethnopharmacol. 2017; 203:214-225. DOI: 10.1016/j.jep.2017.03.048. View