Smooth Muscle Contractility of Laser-enucleated Prostate Tissues and Impacts of Preoperative α-blocker Treatment in Patients with and Without Catheterization

Overview

Journal Sci Rep

Date 2025 Feb 10

PMID 39929919

Authors

Patrick Keller

Sheng Hu

Laurenz Berger

Philip Nicola

Felix Schierholz

Alexander Tamalunas

Oluwafemi E Kale

Philipp Weinhold

Raphaela Waidelich

Christian G Stief

Martin Hennenberg

Affiliations

Soon will be listed here.

Abstract

Prostate smooth muscle contraction is central in treatment of voiding symptoms in benign prostatic hyperplasia (BPH). Tissues from transurethral resection of the prostate (TURP) and radical prostatectomy (RP) for prostate cancer are widely used to study contractions. However, findings are limited by traumatization in TURP, and uncertain relationship to BPH in RP tissues. This study aims to examine contractions of laser-enucleated tissues. Tissues from holmium/thulium laser enucleation (HoLEP/ThuLEP) and TURP were contracted by KCl, noradrenaline and electric field stimulation (EFS) in an organ bath. Contractions were compared to RP tissues in previous studies. KCl-induced contractions averaged 2.5 mN, 0.7 mN and 3.3 mN in tissues from HoLEP/ThuLEP, TURP and RP, with non-responsive tissues included (2.4% HoLEP/ThuLEP, 37% TURP). Maximum EFS-induced contractions (E) averaged 47% of KCl in HoLEP/ThuLEP tissues, 27% in TURP tissues, and 68-235% in 21 previous studies with RP tissues. E values for noradrenaline averaged 99.7% in HoLEP/ThuLEP tissues, 56% in TURP tissues, and ranged from 92 to 260% in RP tissues. Preoperative α-blocker treatment reduced EFS- and noradrenaline-induced contractions, and increased EC values for noradrenaline in laser-enucleated, catheterized patients, but not in patients without catheterization. Also, the ex vivo application of α-blockers increased the EC values for noradrenaline and reduced E for EFS. Laser-enucleated tissues allow investigation of prostate smooth muscle contraction in medication-refractory voiding symptoms. Different impacts of preoperative α-blocker treatment on ex vivo contractility in tissues from patients with and without catheterization point to clinically relevant heterogeneity of patients undergoing surgery for BPH.

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