Acute Toxicity Effects of Ethylene Glycol on Lethal Dose 50 (LD), Urine Production, and Histopathology Change Renal Tubule Cell in Mice

Overview

Journal Open Vet J

Date 2025 Feb 10

PMID 39927377

Authors

Dewa Ketut Meles

Imam Mustofa

Wurlina Wurlina

Clarissa Audreylea Donova

Essi Rayareswari Hidayanti

Niluh Suwasanti

Zulfi Nur Amrina Rosyada

Aswin Rafif Khairullah

Adeyinka Oye Akintunde

Rheza Imawan Mustofa

Satriawan Wedniyanto Putra

Riza Zainuddin Ahmad

Wasito Wasito

Ricadonna Raissa

Affiliations

Soon will be listed here.

Abstract

Background: The LD (lethal dose causing 50% mortality) of ethylene glycol (EG) and its associated toxicity in mice () were assessed by evaluating kidney function.

Aim: This study aimed to determine the acute toxicity of an oral lethal dose of 50% (LD) of EG, also degeneration, necrosis, and inflammatory cell invasion in kidney tubules of male rats () as an animal model.

Methods: There were 66 DDG (Deutschland Denken Yoken) mice in 11 groups of six in this investigation. The daylong metabolic cage study contained one control (C) and 10 treatment groups that received different EG doses. EG's daily BW/kg dosage varied from 0.5 mg/kg to 15,000 mg/kg. The treatment group was given a single dose of EG at a dose of T1 = 0.5 mg/kg BW, T2 = 1.57 mg/kg BW, T3 = 4.94 mg/kg BW, T4 = 15.54 mg/kg BW, T5 = 48.84 mg/kg BW, T6 = 153.55 mg/kg BW, T7 = 482.74 mg/kg BW, and T8 = 1517.66 mg/kg BW T9 = 4771.24 mg/kg BW, T10 = 14999.99 ≈ 15. 000 mg/kg BW. One-way Analysis of Variance testing was used to analyze the data.

Results: The LD value of EG in mice was determined to be 1.598 mg/kg BW, classifying EG as "Slightly Toxic." According to renal histopathology, EG dosage increased renal tubular degeneration, necrosis, and interstitial inflammatory cell infiltration. Acute renal impairment and lower urine output were observed in the EG (4.94 mg/kg-1517.66 mg/kg BW). Histologically, EG levels are associated with renal tubular cell degeneration, necrosis, and interstitial inflammatory cell growth.

Conclusion: Acute EG exposure caused renal failure in male mice. Acute exposure to EG causes renal tubular cell degeneration and inflammation, indicating toxicity and health hazards.

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