Densely Vascularized Thick 3D Tissue Shows Enhanced Protein Secretion Constructed with Intermittent Positive Pressure

Overview

Journal Commun Biol

Date 2025 Feb 8

PMID 39922952

Authors

Misako Katsuura

Jun Homma

Yuhei Higashi

Hidekazu Sekine

Tatsuya Shimizu

Affiliations

Soon will be listed here.

Abstract

Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine.

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