Update on Development of Monoclonal Antibodies for Use in Clinical Flow Cytometry and Research in Dogs

Overview

Journal J Vet Diagn Invest

Date 2025 Feb 6

PMID 39910737

Authors

Cleverson de Souza

Janean Fidel

William C Davis

Affiliations

Soon will be listed here.

Abstract

Since the First International Canine Leukocyte Antigen Workshop in 1994, individual laboratories have worked to expand the repertoire of monoclonal antibodies (mAbs) used in clinical testing and research in dogs. Here, we employed flow cytometry to document the specificity of mAbs submitted to the canine workshop and the animal homologue section of the human HLDA8 international workshop. We also provide an initial characterization of new mAbs derived from hybridomas developed from mice immunized with leukocytes from the blood of a healthy dog and from mice immunized with cells from a dog with B-cell leukemia. These mAbs enhance the tools available for characterizing leukemias, lymphomas, and other hematologic disorders in dogs, as well as for researching the canine immune response to pathogens. Importantly, some of the mAbs submitted to the canine and HLDA8 workshop recognize highly conserved epitopes expressed on orthologues of cluster of differentiation molecules first identified in humans and present an opportunity to develop a cross-species panel for clinical testing and research.

References

Howard C, Morrison W, Bensaid A, Davis W, Eskra L, Gerdes J . Summary of workshop findings for leukocyte antigens of cattle. Vet Immunol Immunopathol. 1991; 27(1-3):21-7. DOI: 10.1016/0165-2427(91)90072-k. View

Hamilton M, Davis W . Culture conditions that optimize outgrowth of hybridomas. Methods Mol Biol. 1995; 45:17-28. DOI: 10.1385/0-89603-308-2:17. View

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

Haverson K, Saalmuller A, Chen Z, Huang C, Simon A, Seebach J . Summary of the first round analyses of the Third International Workshop on Swine Leukocyte Differentiation Antigens. Vet Immunol Immunopathol. 2001; 80(1-2):25-34. DOI: 10.1016/s0165-2427(01)00291-4. View

Davis W, Marusic S, Lewin H, Splitter G, Perryman L, McGuire T . The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet Immunol Immunopathol. 1987; 15(4):337-76. DOI: 10.1016/0165-2427(87)90005-5. View

Cobbold S, Metcalfe S . Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994; 43(3):137-54. DOI: 10.1111/j.1399-0039.1994.tb02315.x. View

Saalmuller A, Lunney J, Daubenberger C, Davis W, Fischer U, Gobel T . Summary of the animal homologue section of HLDA8. Cell Immunol. 2005; 236(1-2):51-8. DOI: 10.1016/j.cellimm.2005.08.009. View

Bernard A, Boumsell L . The clusters of differentiation (CD) defined by the First International Workshop on Human Leucocyte Differentiation Antigens. Hum Immunol. 1984; 11(1):1-10. DOI: 10.1016/0198-8859(84)90051-x. View

Moore P, Rossitto P, Danilenko D, Wielenga J, Raff R, Severns E . Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils. Tissue Antigens. 1992; 40(2):75-85. DOI: 10.1111/j.1399-0039.1992.tb01963.x. View

10.

Schuberth H, Kucinskiene G, Chu R, Faldyna M . Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes. Vet Immunol Immunopathol. 2007; 119(1-2):47-55. DOI: 10.1016/j.vetimm.2007.06.013. View

11.

Lunn D, Holmes M, Antczak D . Summary report of the Second Equine Leucocyte Antigen Workshop. Vet Immunol Immunopathol. 1996; 54(1-4):159-61. DOI: 10.1016/s0165-2427(96)05674-7. View

12.

Allen A, Park K, Barrington G, Lahmers K, Hamilton M, Davis W . Development of a bovine ileal cannulation model to study the immune response and mechanisms of pathogenesis of paratuberculosis. Clin Vaccine Immunol. 2009; 16(4):453-63. PMC: 2668272. DOI: 10.1128/CVI.00347-08. View

13.

Davis W, Davis J, Hamilton M . Use of monoclonal antibodies and flow cytometry to cluster and analyze leukocyte differentiation molecules. Methods Mol Biol. 1995; 45:149-67. DOI: 10.1385/0-89603-308-2:149. View