A Fully Phased Octoploid Strawberry Genome Reveals the Evolutionary Dynamism of Centromeric Satellites

Overview

Journal Genome Biol

Date 2025 Feb 3

PMID 39901151

Authors

Xin Jin

Haiyuan Du

Maoxian Chen

Xu Zheng

Yiying He

Andan Zhu

Affiliations

Soon will be listed here.

Abstract

We systematically examine the application of different phasing strategies to decrypt strawberry genome organization and produce a fully phased and accurate reference genome for Fragaria x ananassa cv. "EA78" (2n = 8x = 56). We identify 147 bp canonical centromeric repeats across 50 strawberry chromosomes and uncover the formation of six neocentromeres through centromere turnover. Our findings indicate strawberry genomes have diverged centromeric satellite arrays among chromosomes, particularly across homoeologs, while maintaining high sequence similarity between homologs. We trace the evolutionary dynamics of centromeric repeats and find substantial centromere size expansion in wild and cultivated octoploids compared to the diploid ancestor, F. vesca.

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