Amphora Coffeiformis Extracellular Polymeric Substances and Their Potential Applications in Lead Removal

Overview

Journal Antonie Van Leeuwenhoek

Publisher Springer

Date 2025 Feb 3

PMID 39899145

Authors

Jihen Elleuch

Marwa Drira

Imtinen Ghribi

Farah Hadjkacem

Guillaume Pierre

Christel Causserand

Hamadi Khemakhem

Philippe Michaud

Imen Fendri

Slim Abdelkafi

Affiliations

Soon will be listed here.

Abstract

Microorganisms producing extracellular polymeric substances (EPS) are of great potential in numerous environmental applications. The present study explores the production and properties of extracellular polymeric substances (EPS) from Amphora coffeiformis diatom strain and their potential applications in environmental remediation. EPS were composed of a complex mixture of polysaccharides, proteins, humic substances and nucleic acids, with polyanionic characteristics as revealed by FTIR, Raman and zeta potential analyses. EPS showed high flocculation efficiency against kaolin clay at low dosages (5 mg/L) through a charge neutralization mechanism involving both polysaccharides and proteins. EPS also exhibited strong emulsification activity for various nonpolar substrates, mainly olive oil, corn oil, soybean oil, essence and diesel, with emulsification indexes above 80%. The emulsions were stable for 72 h under different NaCl concentrations (1-10% w/v). Moreover, EPS demonstrated remarkable adsorption capacity for lead, reaching a maximum of 1699.33 ± 89.61 mg/g under optimized conditions using Box-Behnken design. The adsorption mechanism involved multiple functional groups such as hydroxyl, carbonyl, carboxyl, phosphoric and sulfhydryl. Therefore, EPS from A. coffeiformis are a promising candidate for restoring environments contaminated by heavy metals.

References

Agunbiade M, Pohl C, Ashafa O . Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment. Braz J Microbiol. 2018; 49(4):731-741. PMC: 6175721. DOI: 10.1016/j.bjm.2017.02.013. View

Bala Subramanian S, Yan S, Tyagi R, Surampalli R . Extracellular polymeric substances (EPS) producing bacterial strains of municipal wastewater sludge: isolation, molecular identification, EPS characterization and performance for sludge settling and dewatering. Water Res. 2010; 44(7):2253-66. DOI: 10.1016/j.watres.2009.12.046. View

Bezawada J, Hoang N, More T, Yan S, Tyagi N, Tyagi R . Production of extracellular polymeric substances (EPS) by Serratia sp.1 using wastewater sludge as raw material and flocculation activity of the EPS produced. J Environ Manage. 2013; 128:83-91. DOI: 10.1016/j.jenvman.2013.04.039. View

Bisht V, Lal B . Exploration of Performance Kinetics and Mechanism of Action of a Potential Novel Bioflocculant BF-VB2 on Clay and Dye Wastewater Flocculation. Front Microbiol. 2019; 10:1288. PMC: 6568053. DOI: 10.3389/fmicb.2019.01288. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Chao Y, Zhang T . Surface-enhanced Raman scattering (SERS) revealing chemical variation during biofilm formation: from initial attachment to mature biofilm. Anal Bioanal Chem. 2012; 404(5):1465-75. PMC: 3426672. DOI: 10.1007/s00216-012-6225-y. View

Cheah Y, Chan D . A methodological review on the characterization of microalgal biofilm and its extracellular polymeric substances. J Appl Microbiol. 2022; 132(5):3490-3514. DOI: 10.1111/jam.15455. View

Costa O, Raaijmakers J, Kuramae E . Microbial Extracellular Polymeric Substances: Ecological Function and Impact on Soil Aggregation. Front Microbiol. 2018; 9:1636. PMC: 6064872. DOI: 10.3389/fmicb.2018.01636. View

Cunha C, Silva L, Paulo J, Faria M, Nogueira N, Cordeiro N . Microalgal-based biopolymer for nano- and microplastic removal: a possible biosolution for wastewater treatment. Environ Pollut. 2020; 263(Pt B):114385. DOI: 10.1016/j.envpol.2020.114385. View

10.

Das S, Sen I, Kati A, Some S, Mandal A, Islam S . Flocculating, emulsification and metal sorption properties of a partial characterized novel exopolysaccharide produced by Rhizobium tropici SRA1 isolated from Psophocarpus tetragonolobus (L) D.C. Int Microbiol. 2019; 22(1):91-101. DOI: 10.1007/s10123-018-0031-0. View

11.

Delattre C, Pierre G, Laroche C, Michaud P . Production, extraction and characterization of microalgal and cyanobacterial exopolysaccharides. Biotechnol Adv. 2016; 34(7):1159-1179. DOI: 10.1016/j.biotechadv.2016.08.001. View

12.

Di Martino P . Extracellular polymeric substances, a key element in understanding biofilm phenotype. AIMS Microbiol. 2019; 4(2):274-288. PMC: 6604936. DOI: 10.3934/microbiol.2018.2.274. View

13.

Ding R, Luo L, Han R, Zhang M, Li T, Tang J . Rapid Production of a Novel Al(III) Dependent Bioflocculant Isolated From 160-1 and Its Application Combined With Inorganic Salts. Front Microbiol. 2021; 11:622365. PMC: 7835285. DOI: 10.3389/fmicb.2020.622365. View

14.

Dong B, Liu G, Zhou J, Cai L, Wang J, Jin R . Roles of molecular weight-fractionated extracellular polymeric substance in transformation of Au(III) to Au nanoparticles in aqueous environments. Sci Total Environ. 2020; 728:138889. DOI: 10.1016/j.scitotenv.2020.138889. View

15.

Drira M, Elleuch J, Ben Hlima H, Hentati F, Gardarin C, Rihouey C . Optimization of Exopolysaccharides Production by and Their Potential to Induce Defense Responses in against . Biomolecules. 2021; 11(2). PMC: 7918794. DOI: 10.3390/biom11020282. View

16.

Drira M, Elleuch J, Hadjkacem F, Hentati F, Drira R, Pierre G . Influence of the sulfate content of the exopolysaccharides from Porphyridium sordidum on their elicitor activities on date palm vitroplants. Plant Physiol Biochem. 2022; 186:99-106. DOI: 10.1016/j.plaphy.2022.06.012. View

17.

Elleuch J, Ben Amor F, Chaaben Z, Frikha F, Michaud P, Fendri I . Zinc biosorption by Dunaliella sp. AL-1: Mechanism and effects on cell metabolism. Sci Total Environ. 2021; 773:145024. DOI: 10.1016/j.scitotenv.2021.145024. View

18.

Elleuch J, Hmani R, Drira M, Michaud P, Fendri I, Abdelkafi S . Potential of three local marine microalgae from Tunisian coasts for cadmium, lead and chromium removals. Sci Total Environ. 2021; 799:149464. DOI: 10.1016/j.scitotenv.2021.149464. View

19.

Elleuch J, Hadj Kacem F, Ben Amor F, Hadrich B, Michaud P, Fendri I . Extracellular neutral protease from Arthrospira platensis: Production, optimization and partial characterization. Int J Biol Macromol. 2020; 167:1491-1498. DOI: 10.1016/j.ijbiomac.2020.11.102. View

20.

Elleuch J, Thabet J, Ghribi I, Jabeur H, Hernandez L, Fendri I . Responses of Dunaliella sp. AL-1 to chromium and copper: Biochemical and physiological studies. Chemosphere. 2024; 364:143133. DOI: 10.1016/j.chemosphere.2024.143133. View