Nuclear Talin-1 Provides a Bridge Between Cell Adhesion and Gene Expression

Overview

Journal iScience

Publisher Cell Press

Date 2025 Feb 3

PMID 39898029

Authors

Alejandro J Da Silva

Hendrik S E Hastbacka

Mikael C Puustinen

Jenny C Pessa

Jens C Luoto

Erika Sundstrom

Benjamin T Goult

Guillaume Jacquemet

Eva Henriksson

Lea Sistonen

Affiliations

Soon will be listed here.

Abstract

Talin-1 (TLN1) is best known to activate integrin receptors and transmit mechanical stimuli to the actin cytoskeleton at focal adhesions. However, the localization of TLN1 is not restricted to focal adhesions. By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. Importantly, small interfering RNA (siRNA)-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. To determine the functional impact of nuclear TLN1, we expressed a TLN1 fusion protein containing a nuclear localization signal. Our findings revealed that the accumulation of nuclear TLN1 alters the expression of a subset of genes and impairs the formation of cell-cell clusters. This study introduces an additional perspective on the canonical view of TLN1 subcellular localization and function.

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