Preparation of Flavonoids from and Evaluation of Their Antioxidant and α-glucosidase Inhibitory Activities

Overview

Journal Food Chem X

Date 2025 Feb 3

PMID 39897968

Authors

Zelin Huang

Yan Zhao

Weixing Yang

Lu Lang

Jun Sheng

Yang Tian

Xiaoyu Gao

Affiliations

Soon will be listed here.

Abstract

is an important homologous medicinal and food plant, and its fruit is rich in flavonoids. However, few studies have reported the preparation and bioactivity of flavonoids in (ATF). In this study, the optimal conditions for ultrasound-assisted extraction of ATF were identified through response surface optimization. HPD300 was identified as the best resin for the purification of ATF, as it exhibited a Freundlich model-conformative adsorption isotherm. Among the different concentrations of ethanol, 20 % and 30 % resulted in higher flavonoid purity (>90 %) and stronger antioxidant and α-glucosidase inhibition activities. A widely targeted metabolomics assay revealed that the relative abundance of flavonoids in a mixture of 20 % and 30 % ethanol eluates was greater than 73 %, which mainly contained (+)-epicatechin, isoquercitrin, astragalin kaempferol-3-O-rutinoside, and procyanidin B2. These findings provide a theoretical basis for the in-depth development and potential use of ATF in the functional food, cosmetic and pharmaceutical industries.

Citing Articles

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Liu L, Zhao Y, Yang W, Han L, Mo X, Sheng J Int J Mol Sci. 2025; 26(4).

PMID: 40004140 PMC: 11855768. DOI: 10.3390/ijms26041676.

References

Andrade A, Machado K, Machado K, Dias Ribeiro Figueiredo D, David J, Islam M . In vitro antioxidant properties of the biflavonoid agathisflavone. Chem Cent J. 2018; 12(1):75. PMC: 6026112. DOI: 10.1186/s13065-018-0443-0. View

Hu Y, Gao X, Zhao Y, Liu S, Luo K, Fu X . Flavonoids in Crevost et Lemarie Ameliorate Loperamide-Induced Constipation in Mice by Regulating Gut Microbiota and Related Metabolites. Int J Mol Sci. 2023; 24(8). PMC: 10139007. DOI: 10.3390/ijms24087191. View

Vo T, Pham N, Pham T, Nguyen H, Vo L, Tran T . Green extraction of total phenolic and flavonoid contents from mangosteen ( L) rind using natural deep eutectic solvents. Heliyon. 2023; 9(4):e14884. PMC: 10121615. DOI: 10.1016/j.heliyon.2023.e14884. View

Hu C, Wong W, Wu R, Lai W . Biochemistry and use of soybean isoflavones in functional food development. Crit Rev Food Sci Nutr. 2019; 60(12):2098-2112. DOI: 10.1080/10408398.2019.1630598. View

Cai R, Yue X, Wang Y, Yang Y, Sun D, Li H . Chemistry and bioactivity of plants from the genus Amomum. J Ethnopharmacol. 2021; 281:114563. DOI: 10.1016/j.jep.2021.114563. View

Calzada F, Solares-Pascasio J, Ordonez-Razo R, Velazquez C, Barbosa E, Garcia-Hernandez N . Antihyperglycemic Activity of the Leaves from Miller and Rutin on Alloxan-induced Diabetic Rats. Pharmacognosy Res. 2017; 9(1):1-6. PMC: 5330092. DOI: 10.4103/0974-8490.199781. View

Hua F, Zhou P, Liu P, Bao G . Rat plasma protein binding of kaempferol-3-O-rutinoside from Lu'an GuaPian tea and its anti-inflammatory mechanism for cardiovascular protection. J Food Biochem. 2021; 45(7):e13749. DOI: 10.1111/jfbc.13749. View

Yi X, Dong M, Guo N, Tian J, Lei P, Wang S . Flavonoids improve type 2 diabetes mellitus and its complications: a review. Front Nutr. 2023; 10:1192131. PMC: 10265523. DOI: 10.3389/fnut.2023.1192131. View

Qin G, Zhang F, Ren M, Chen X, Liu C, Li G . Eco-friendly and efficient extraction of polyphenols from Ligustrum robustum by deep eutectic solvent assisted ultrasound. Food Chem. 2023; 429:136828. DOI: 10.1016/j.foodchem.2023.136828. View

10.

Aksoy A, Sandikci Altunatmaz S, Aksu F, Tokatli Demirok N, Yazici K, Yikmis S . Bee Bread as a Functional Product: Phenolic Compounds, Amino Acid, Sugar, and Organic Acid Profiles. Foods. 2024; 13(5). PMC: 10931289. DOI: 10.3390/foods13050795. View

11.

Hermankova-Vavrikova E, Krenkova A, Petraskova L, Chambers C, Zapal J, Kuzma M . Synthesis and Antiradical Activity of Isoquercitrin Esters with Aromatic Acids and Their Homologues. Int J Mol Sci. 2017; 18(5). PMC: 5454983. DOI: 10.3390/ijms18051074. View

12.

Li H, Yang J, Wang M, Ma X, Peng X . Studies on the inhibition of α-glucosidase by biflavonoids and their interaction mechanisms. Food Chem. 2023; 420:136113. DOI: 10.1016/j.foodchem.2023.136113. View

13.

Yang Y, Liang Q, Zhang B, Zhang J, Fan L, Kang J . Adsorption and desorption characteristics of flavonoids from white tea using macroporous adsorption resin. J Chromatogr A. 2024; 1715:464621. DOI: 10.1016/j.chroma.2023.464621. View

14.

Cui L, Ma Z, Wang D, Niu Y . Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves. Ultrason Sonochem. 2022; 90:106190. PMC: 9554832. DOI: 10.1016/j.ultsonch.2022.106190. View

15.

Xie Y, Guo Q, Wang G . Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins. Molecules. 2016; 21(6). PMC: 6273629. DOI: 10.3390/molecules21060768. View

16.

Maduwanthi S, Marapana R . Total phenolics, flavonoids and antioxidant activity following simulated gastro-intestinal digestion and dialysis of banana (Musa acuminata, AAB) as affected by induced ripening agents. Food Chem. 2020; 339:127909. DOI: 10.1016/j.foodchem.2020.127909. View

17.

Nagarajan S, Nagarajan R, Kumar J, Salemme A, Togna A, Saso L . Antioxidant Activity of Synthetic Polymers of Phenolic Compounds. Polymers (Basel). 2020; 12(8). PMC: 7464737. DOI: 10.3390/polym12081646. View

18.

Lin Y, Lu M, Liao H, Li Y, Han W, Yuan K . Content determination of the flavonoids in the different parts and different species of Abelmoschus esculentus L. by reversed phase-high performance liquid chromatograph and colorimetric method. Pharmacogn Mag. 2014; 10(39):278-84. PMC: 4159921. DOI: 10.4103/0973-1296.137368. View

19.

Li X, Tian Y, Wang T, Lin Q, Feng X, Jiang Q . Role of the p-Coumaroyl Moiety in the Antioxidant and Cytoprotective Effects of Flavonoid Glycosides: Comparison of Astragalin and Tiliroside. Molecules. 2017; 22(7). PMC: 6152332. DOI: 10.3390/molecules22071165. View

20.

Shen D, Labreche F, Wu C, Fan G, Li T, Dou J . Ultrasound-assisted adsorption/desorption of jujube peel flavonoids using macroporous resins. Food Chem. 2021; 368:130800. DOI: 10.1016/j.foodchem.2021.130800. View