Metabolic Fate of Chokeberry () Phenolics in Different Food Matrices

Overview

Journal Curr Res Food Sci

Date 2025 Feb 3

PMID 39896272

Authors

Magdalena Kopsel

Gulay Ozkan

Tuba Esatbeyoglu

Affiliations

Soon will be listed here.

Abstract

Chokeberry () has been traditionally used as a folk remedy due to its health-promoting effects. The aim of this study was to investigate the effects of chokeberry polyphenols combined with the matrices of milk and milk alternatives on the permeability of the intestinal barrier. Based on this, availability of chokeberry polyphenols was tested by gastrointestinal model combined with a co-culture of human colon adenocarcinoma cells (Caco-2) and human colon cancer cells (HT29-MTX). Additionally, the antioxidant capacity of the samples was analyzed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. According to the results, both chokeberry juice and chokeberry juice in combination with milk showed a higher recovery of DPPH radical scavenging ability after intestinal digestion. Moreover, a significant difference in the transport of Lucifer Yellow through the intestinal membrane was observed when compared to the control. Therefore, fat- and protein-rich food matrices could represent a potential to increase the bioavailability of phenolic compounds while reducing intestinal barrier injury.

References

Carrasco-Pozo C, Morales P, Gotteland M . Polyphenols protect the epithelial barrier function of Caco-2 cells exposed to indomethacin through the modulation of occludin and zonula occludens-1 expression. J Agric Food Chem. 2013; 61(22):5291-7. DOI: 10.1021/jf400150p. View

Brodkorb A, Egger L, Alminger M, Alvito P, Assuncao R, Ballance S . INFOGEST static in vitro simulation of gastrointestinal food digestion. Nat Protoc. 2019; 14(4):991-1014. DOI: 10.1038/s41596-018-0119-1. View

Swieca M, Seczyk L, Gawlik-Dziki U, Dziki D . Bread enriched with quinoa leaves - the influence of protein-phenolics interactions on the nutritional and antioxidant quality. Food Chem. 2014; 162:54-62. DOI: 10.1016/j.foodchem.2014.04.044. View

Adolfsson O, Meydani S, Russell R . Yogurt and gut function. Am J Clin Nutr. 2004; 80(2):245-56. DOI: 10.1093/ajcn/80.2.245. View

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M . Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231-7. DOI: 10.1016/s0891-5849(98)00315-3. View

Ozkan G, Kostka T, Drager G, Capanoglu E, Esatbeyoglu T . Bioaccessibility and transepithelial transportation of cranberrybush (Viburnum opulus) phenolics: Effects of non-thermal processing and food matrix. Food Chem. 2022; 380:132036. DOI: 10.1016/j.foodchem.2021.132036. View

Buniowska M, Carbonell-Capella J, Frigola A, Esteve M . Bioaccessibility of bioactive compounds after non-thermal processing of an exotic fruit juice blend sweetened with Stevia rebaudiana. Food Chem. 2016; 221:1834-1842. DOI: 10.1016/j.foodchem.2016.10.093. View

Suzuki T, Hara H . Quercetin enhances intestinal barrier function through the assembly of zonula [corrected] occludens-2, occludin, and claudin-1 and the expression of claudin-4 in Caco-2 cells. J Nutr. 2009; 139(5):965-74. DOI: 10.3945/jn.108.100867. View

Fresco P, Borges F, Diniz C, Marques M . New insights on the anticancer properties of dietary polyphenols. Med Res Rev. 2006; 26(6):747-66. DOI: 10.1002/med.20060. View

10.

Dima C, Assadpour E, Dima S, Jafari S . Bioavailability and bioaccessibility of food bioactive compounds; overview and assessment by in vitro methods. Compr Rev Food Sci Food Saf. 2020; 19(6):2862-2884. DOI: 10.1111/1541-4337.12623. View

11.

Manganaris G, Goulas V, Vicente A, Terry L . Berry antioxidants: small fruits providing large benefits. J Sci Food Agric. 2013; 94(5):825-33. DOI: 10.1002/jsfa.6432. View

12.

Meydani S, Ha W . Immunologic effects of yogurt. Am J Clin Nutr. 2000; 71(4):861-72. DOI: 10.1093/ajcn/71.4.861. View

13.

Wolosiak R, Druzynska B, Derewiaka D, Piecyk M, Majewska E, Ciecierska M . Verification of the Conditions for Determination of Antioxidant Activity by ABTS and DPPH Assays-A Practical Approach. Molecules. 2022; 27(1). PMC: 8747050. DOI: 10.3390/molecules27010050. View

14.

Cui W, Li L, Sun C, Wen Y, Zhou Y, Dong Y . Tumor necrosis factor alpha increases epithelial barrier permeability by disrupting tight junctions in Caco-2 cells. Braz J Med Biol Res. 2010; 43(4):330-7. DOI: 10.1590/S0100-879X2010007500020. View

15.

Csepregi K, Neugart S, Schreiner M, Hideg E . Comparative Evaluation of Total Antioxidant Capacities of Plant Polyphenols. Molecules. 2016; 21(2). PMC: 6274360. DOI: 10.3390/molecules21020208. View

16.

Astrup A . Yogurt and dairy product consumption to prevent cardiometabolic diseases: epidemiologic and experimental studies. Am J Clin Nutr. 2014; 99(5 Suppl):1235S-42S. DOI: 10.3945/ajcn.113.073015. View

17.

Costa J, Ahluwalia A . Advances and Current Challenges in Intestinal Model Engineering: A Digest. Front Bioeng Biotechnol. 2019; 7:144. PMC: 6591368. DOI: 10.3389/fbioe.2019.00144. View

18.

Goslinski M, Nowak D, Szwengiel A . Multidimensional Comparative Analysis of Bioactive Phenolic Compounds of Honeys of Various Origin. Antioxidants (Basel). 2021; 10(4). PMC: 8065985. DOI: 10.3390/antiox10040530. View

19.

Rafiee Z, Nejatian M, Daeihamed M, Jafari S . Application of different nanocarriers for encapsulation of curcumin. Crit Rev Food Sci Nutr. 2018; 59(21):3468-3497. DOI: 10.1080/10408398.2018.1495174. View

20.

Antunes F, Andrade F, Araujo F, Ferreira D, Sarmento B . Establishment of a triple co-culture in vitro cell models to study intestinal absorption of peptide drugs. Eur J Pharm Biopharm. 2012; 83(3):427-35. DOI: 10.1016/j.ejpb.2012.10.003. View