Endophytic Fungi Isolated from the Marine Macroalga in Korea

Overview

Journal Mycobiology

Date 2025 Feb 3

PMID 39895928

Authors

Ji-Won Kim

Yun-Jeong Kim

Ahn-Heum Eom

Affiliations

Soon will be listed here.

Abstract

The exploration of endophytic fungi associated with seaweeds has garnered significant interest due to their crucial ecological functions and potential as sources of valuable bioactive compounds. In this study, we isolated and identified endophytic fungi from the brown seaweed , collected from the intertidal zone of Yeongdoek, Gyeongsangbuk-do in Korea. Through morphological examination and molecular phylogenetic analysis using multiple molecular markers, including ITS, LSU, SSU, TEF1, TUB2, and RPB2 sequences, we identified three fungal species not previously recorded in Korea: , , and . Detailed descriptions of morphological characteristics and phylogenetic analyses are provided. This study represents the first report of these endophytic fungi isolated from in Korea, thereby enhancing the understanding of the diversity of seaweed-associated endophytic fungi in the region.

References

Kim J, Kim Y, Eom A . Diversity and Community Structure of Endophytic Fungi Isolated from the Brown Seaweed in Coastal Regions of Korea. Mycobiology. 2024; 52(5):317-323. PMC: 11619010. DOI: 10.1080/12298093.2024.2416730. View

Kuvarina A, Gavryushina I, Sykonnikov M, Efimenko T, Markelova N, Bilanenko E . Exploring Peptaibol's Profile, Antifungal, and Antitumor Activity of Emericellipsin A of Species from Soda and Saline Soils. Molecules. 2022; 27(5). PMC: 8911692. DOI: 10.3390/molecules27051736. View

Lee J, Lee W, Perera R, Lim Y . Long-Term Investigation of Marine-Derived Diversity in the Republic of Korea. Mycobiology. 2024; 51(6):436-444. PMC: 10763834. DOI: 10.1080/12298093.2023.2279342. View

Flewelling A, Johnson J, Gray C . Antimicrobials from the marine algal endophyte Penicillium sp. Nat Prod Commun. 2013; 8(3):373-4. View

Gardes M, Bruns T . ITS primers with enhanced specificity for basidiomycetes--application to the identification of mycorrhizae and rusts. Mol Ecol. 1993; 2(2):113-8. DOI: 10.1111/j.1365-294x.1993.tb00005.x. View

Sahoo S, Subban K, Chelliah J . Diversity of Marine Macro-Algicolous Endophytic Fungi and Cytotoxic Potential of Metabolites Against Cancer Cell Lines. Front Microbiol. 2021; 12:650177. PMC: 8236939. DOI: 10.3389/fmicb.2021.650177. View

Glass N, Donaldson G . Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995; 61(4):1323-30. PMC: 167388. DOI: 10.1128/aem.61.4.1323-1330.1995. View

Tan R, Zou W . Endophytes: a rich source of functional metabolites. Nat Prod Rep. 2001; 18(4):448-59. DOI: 10.1039/b100918o. View

Singh R, Kumari P, Reddy C . Antimicrobial compounds from seaweeds-associated bacteria and fungi. Appl Microbiol Biotechnol. 2015; 99(4):1571-86. DOI: 10.1007/s00253-014-6334-y. View

10.

Tamura K, Stecher G, Kumar S . MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 2021; 38(7):3022-3027. PMC: 8233496. DOI: 10.1093/molbev/msab120. View

11.

Hou L, Giraldo A, Groenewald J, Rama T, Summerbell R, Huang G . Redisposition of acremonium-like fungi in . Stud Mycol. 2024; 105:23-203. PMC: 11182610. DOI: 10.3114/sim.2023.105.02. View

12.

Jiang N, Voglmayr H, Ma C, Xue H, Piao C, Li Y . A new -like genus of Amphisphaeriales in China. MycoKeys. 2023; 92:27-43. PMC: 9849059. DOI: 10.3897/mycokeys.92.86521. View

13.

Calado M, Silva J, Alves C, Susano P, Santos D, Alves J . Marine endophytic fungi associated with Halopteris scoparia (Linnaeus) Sauvageau as producers of bioactive secondary metabolites with potential dermocosmetic application. PLoS One. 2021; 16(5):e0250954. PMC: 8118457. DOI: 10.1371/journal.pone.0250954. View

14.

Huskisson E, Maggini S, Ruf M . The role of vitamins and minerals in energy metabolism and well-being. J Int Med Res. 2007; 35(3):277-89. DOI: 10.1177/147323000703500301. View

15.

Kjer J, Debbab A, Aly A, Proksch P . Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. Nat Protoc. 2010; 5(3):479-90. DOI: 10.1038/nprot.2009.233. View

16.

Rodriguez R, White Jr J, Arnold A, Redman R . Fungal endophytes: diversity and functional roles. New Phytol. 2009; 182(2):314-330. DOI: 10.1111/j.1469-8137.2009.02773.x. View

17.

Eo J, Choi J, Eom A . Diversity, Distribution, and Host Plant of Endophytic Fungi: A Focus on Korea. Mycobiology. 2023; 50(6):399-407. PMC: 9848380. DOI: 10.1080/12298093.2022.2154044. View

18.

Lee W, Kim D, Perera R, Kim J, Cho Y, Lee J . Diversity of (, ) Species Identified in Korean Macroalgae Including Five Unrecorded Species. Mycobiology. 2024; 51(6):401-409. PMC: 10763912. DOI: 10.1080/12298093.2023.2283272. View

19.

Mani V, Parimala Gnana Soundari A, Karthiyaini D, Preeth K . Bioprospecting Endophytic Fungi and Their Metabolites from Medicinal Tree Aegle marmelos in Western Ghats, India. Mycobiology. 2015; 43(3):303-10. PMC: 4630437. DOI: 10.5941/MYCO.2015.43.3.303. View

20.

Sarasan M, Job N, Puthumana J, R R, M P P, Thomas L . Exploration and profiling of hidden endophytic mycota of marine macroalgae with potential drug leads. FEMS Microbiol Lett. 2020; 367(11). DOI: 10.1093/femsle/fnaa078. View