Sensitive Electrochemical Immunosensor for Procymidone Detection Based on a Supramolecular Amplification Strategy

Overview

Journal ACS Omega

Publisher American Chemical Society

Date 2025 Feb 3

PMID 39895707

Authors

Qiushuang Ai

Xiren Yu

Yifan Dong

Li Zhang

Jingtian Liang

Dawen Zhang

Suyan Qiu

Affiliations

Soon will be listed here.

Abstract

A sensitive electrochemical immunosensor for procymidone detection was developed based on a supramolecular amplification strategy. β-Cyclodextrin (β-CD)-based nanomaterials were employed to immobilize ferrocene derivative (FC)-functionalized antibodies/antigens through host-guest interactions. With the presence of procymidone, the formed β-CD-labeled bioconjugates were immobilized on the antibody-modified electrode after the immunoreaction, indicating fabrication of the immunosensor. The FC/β-CD complexes were with multiplex electroactive species and provided more sites for recognition groups, resulting in signal amplification of the sensor. Monitored with differential pulse voltammetry, the proposed immunosensor exhibited a wide linear range from 5 pM to 0.1 μM with a low detection limit (LOD) of 1.67 pM. The as-prepared immunosensor possessed high sensitivity, specificity, and stability and showed great potential for monitoring procymidone in the field of food safety.

References

Zeng R, Qiu M, Wan Q, Huang Z, Liu X, Tang D . Smartphone-Based Electrochemical Immunoassay for Point-of-Care Detection of SARS-CoV-2 Nucleocapsid Protein. Anal Chem. 2022; 94(43):15155-15161. DOI: 10.1021/acs.analchem.2c03606. View

Wu A, Yu Q, Lu H, Lou Z, Zhao Y, Luo T . Developmental toxicity of procymidone to larval zebrafish based on physiological and transcriptomic analysis. Comp Biochem Physiol C Toxicol Pharmacol. 2021; 248:109081. DOI: 10.1016/j.cbpc.2021.109081. View

Liu Y, Liu Y, Liu Z, Du F, Qin G, Li G . Supramolecularly imprinted polymeric solid phase microextraction coatings for synergetic recognition nitrophenols and bisphenol A. J Hazard Mater. 2019; 368:358-364. DOI: 10.1016/j.jhazmat.2019.01.039. View

Zhang Y, Qi X, Zhang X, Huang Y, Ma Q, Guo X . β-Cyclodextrin/carbon dots-grafted cellulose nanofibrils hydrogel for enhanced adsorption and fluorescence detection of levofloxacin. Carbohydr Polym. 2024; 340:122306. DOI: 10.1016/j.carbpol.2024.122306. View

Mei X, Wang X, Huang W, Zhu J, Liu K, Wang X . A novel polycaprolactone/polypyrrole/β-cyclodextrin electrochemical flexible sensor for dinotefuran pesticide detection. Food Chem. 2023; 434:137194. DOI: 10.1016/j.foodchem.2023.137194. View

Shen W, Zhuo Y, Chai Y, Yang Z, Han J, Yuan R . Enzyme-free electrochemical immunosensor based on host-guest nanonets catalyzing amplification for procalcitonin detection. ACS Appl Mater Interfaces. 2015; 7(7):4127-34. DOI: 10.1021/am508137t. View

Zhao Y, Huang Y, Zhu H, Zhu Q, Xia Y . Three-in-One: Sensing, Self-Assembly, and Cascade Catalysis of Cyclodextrin Modified Gold Nanoparticles. J Am Chem Soc. 2016; 138(51):16645-16654. DOI: 10.1021/jacs.6b07590. View

Ozdogan N, Kapukiran F, Mutluoglu G, Chormey D, Bakirdere S . Simultaneous determination of iprodione, procymidone, and chlorflurenol in lake water and wastewater matrices by GC-MS after multivariate optimization of binary dispersive liquid-liquid microextraction. Environ Monit Assess. 2018; 190(10):607. DOI: 10.1007/s10661-018-6961-3. View

Lin S, Han Y, Jiangyuan C, Luo Y, Xu W, Luo H . Revealing the biodiversity and the response of pathogen to a combined use of procymidone and thiamethoxam in tomatoes. Food Chem. 2019; 284:73-79. DOI: 10.1016/j.foodchem.2019.01.094. View

10.

Kou X, Gao N, Xu X, Zhu J, Ke Q, Meng Q . Preparation, structural analysis of alcohol aroma compounds/β-cyclodextrin inclusion complexes and the application in strawberry preservation. Food Chem. 2024; 457:140160. DOI: 10.1016/j.foodchem.2024.140160. View

11.

Gao Y, Li M, Zeng Y, Liu X, Tang D . Tunable Competitive Absorption-Induced Signal-On Photoelectrochemical Immunoassay for Cardiac Troponin I Based on Z-Scheme Metal-Organic Framework Heterojunctions. Anal Chem. 2022; 94(39):13582-13589. DOI: 10.1021/acs.analchem.2c03263. View

12.

Abe J, Tomigahara Y, Tarui H, Omori R, Kawamura S . Identification of Metabolism and Excretion Differences of Procymidone between Rats and Humans Using Chimeric Mice: Implications for Differential Developmental Toxicity. J Agric Food Chem. 2018; 66(8):1955-1963. DOI: 10.1021/acs.jafc.7b05463. View

13.

Ma T, Chang S, He J, Liang F . Emerging sensing platforms based on Cucurbit[]uril functionalized gold nanoparticles and electrodes. Chem Commun (Camb). 2023; 60(2):150-167. DOI: 10.1039/d3cc04851a. View

14.

Lai Q, Sun X, Li L, Li D, Wang M, Shi H . Toxicity effects of procymidone, iprodione and their metabolite of 3,5-dichloroaniline to zebrafish. Chemosphere. 2021; 272:129577. DOI: 10.1016/j.chemosphere.2021.129577. View

15.

Ning S, Zhou M, Liu C, Waterhouse G, Dong J, Ai S . Ultrasensitive electrochemical immunosensor for avian leukosis virus detection based on a β-cyclodextrin-nanogold-ferrocene host-guest label for signal amplification. Anal Chim Acta. 2019; 1062:87-93. DOI: 10.1016/j.aca.2019.02.041. View

16.

Yu Z, Gong H, Xu J, Li Y, Xue F, Zeng Y . Liposome-Embedded CuAgS Nanoparticle-Mediated Photothermal Immunoassay for Daily Monitoring of cTnI Protein Using a Portable Thermal Imager. Anal Chem. 2022; 94(20):7408-7416. DOI: 10.1021/acs.analchem.2c01133. View

17.

Rahimizadeh K, Zahra Q, Chen S, Le B, Ullah I, Veedu R . Nanoparticles-assisted aptamer biosensing for the detection of environmental pathogens. Environ Res. 2023; 238(Pt 1):117123. DOI: 10.1016/j.envres.2023.117123. View

18.

Cai G, Yu Z, Ren R, Tang D . Exciton-Plasmon Interaction between AuNPs/Graphene Nanohybrids and CdS Quantum Dots/TiO for Photoelectrochemical Aptasensing of Prostate-Specific Antigen. ACS Sens. 2018; 3(3):632-639. DOI: 10.1021/acssensors.7b00899. View

19.

Wu D, Tang J, Yu Z, Gao Y, Zeng Y, Tang D . Pt/Zn-TCPP Nanozyme-Based Flexible Immunoassay for Dual-Mode Pressure-Temperature Monitoring of Low-Abundance Proteins. Anal Chem. 2024; 96(21):8740-8746. DOI: 10.1021/acs.analchem.4c01059. View

20.

Ai Q, Jin L, Gong Z, Liang F . Observing Host-Guest Interactions at Molecular Interfaces by Monitoring the Electrochemical Current. ACS Omega. 2020; 5(18):10581-10585. PMC: 7227043. DOI: 10.1021/acsomega.0c01077. View