Microglial C/EBPβ-Fcgr1 Regulatory Axis Blocking Inhibits Microglial Pyroptosis and Improves Neurological Recovery

Overview

Journal J Neuroinflammation

Publisher Biomed Central

Date 2025 Jan 31

PMID 39891259

Authors

Jing Li

Yubing Yang

Chenguang Zhao

Jinghao Zhao

Xiaohui Wang

Shengshou Ye

Dong Wang

Chengdong Zhou

Jie Li

Shuang Wang

Ke Li

Chunmiao Liu

Xijing He

Jie Qin

Affiliations

Soon will be listed here.

Abstract

CAAT/Enhancer Binding Protein β (C/EBPβ) is associated with inflammatory responses in neurodegenerative pathologies, particularly in the brain. However, the regulatory role of C/EBPβ in spinal cord injury and its impact on neurological recovery remain unknown. In this study, we observed significant upregulation of C/EBPβ in microglia after spinal cord injury in mice and was associated with neuroinflammation. Knocking down C/EBPβ in the spinal cord attenuated microglia pyroptosis, reduced the production of proinflammatory cytokines, and inhibited neuronal apoptosis. Mechanistically, C/EBPβ promoted the transcription of Fcgr1, which was involved in activating microglia pyroptosis. In both in-vivo and in-vitro experiments, knocking down Cebpb or Fcgr1, or the pyroptosis inhibitor VX765 inhibited neuronal apoptosis and improved neurological recovery in mice. These findings indicate that C/EBPβ functions as a key regulator that participates in the microglia pyroptosis-mediated neuroinflammation by activating Fcgr1 transcription.

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