DNA Nanotags for Multiplexed Single-Particle Electron Microscopy and Electron Cryotomography

Overview

Journal JACS Au

Publisher American Chemical Society

Date 2025 Jan 31

PMID 39886579

Authors

Yuanfang Chen

Yiqian Huang

Yuhe R Yang

Affiliations

Soon will be listed here.

Abstract

DNA nanostructures present new opportunities as Nanotags for electron microscopy (EM) imaging, leveraging their high programmability, unique shapes, biomolecule conjugation capability, and stability compatible with standard cryogenic sample preparation protocols. This perspective highlights the potential of DNA Nanotags to enable high-throughput multiplexed EM analysis and facilitate particle identification for cryogenic electron tomography (cryo-ET). Meanwhile, applying Nanotags in live-cell environments requires the efficient cellular uptake of intact structures and successful cytosolic migration. Promising strategies such as employing direct cytosolic delivery platforms and expressing RNA-based Nanotags are discussed, while more systematic studies are needed to fully understand the intracellular trafficking and achieve precise localization of DNA Nanotags.

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