The TCP Transcription Factor TAC8 Positively Regulates the Tiller Angle in Rice (Oryza Sativa L.)

Overview

Journal Theor Appl Genet

Publisher Springer

Date 2025 Jan 30

PMID 39885061

Authors

Yuntao Yan

Ya Chen

Xiaoya Zhu

Yan Wang

Hui Qi

Jinxin Gui

Haiqing Zhang

Jiwai He

Affiliations

Soon will be listed here.

Abstract

The tiller angle, one of the critical factors that determine the rice plant type, is closely related to rice yield. An appropriate rice tiller angle can improve rice photosynthetic efficiency and increase yields. In this study, we identified a transcription factor, TILLRE ANGLE CONTROL 8 (TAC8), that is highly expressed in the rice tiller base and positively regulates the tiller angle by regulating cell length and endogenous auxin content; TAC8 encodes a TEOSINTE BRANCHED1/CYCLOIDEA/PCF transcriptional activator that is highly expressed in the nucleus. RNA-seq revealed that TAC8 is involved mainly in the photoperiod and abiotic stress response in rice. Yeast two-hybrid assays verified that TAC8 interacts with CHLOROPHYLL A/B-BINDING PROTEIN 1, which responds to photoperiod, and haplotype analysis revealed that a 34-bp deletion at position 1516 in the promoter region and a 9-bp deletion at position 153 in the coding region can result in impaired function or loss of function of TAC8. This study provides a new genetic resource for designing ideal plant types with appropriate rice tiller angle.

Citing Articles

Genome-Wide Exploration and Characterization of the Gene Family's Expression Patterns in Response to Abiotic Stresses in Siberian Wildrye ( L.).

Liu T, Peng J, Dong Z, Liu Y, Wu J, Xiong Y Int J Mol Sci. 2025; 26(5).

PMID: 40076552 PMC: 11900556. DOI: 10.3390/ijms26051925.

References

Cai Y, Huang L, Song Y, Yuan Y, Xu S, Wang X . LAZY3 interacts with LAZY2 to regulate tiller angle by modulating shoot gravity perception in rice. Plant Biotechnol J. 2023; 21(6):1217-1228. PMC: 10214757. DOI: 10.1111/pbi.14031. View

Chen S, Zhou L, Xu P, Xue H . SPOC domain-containing protein Leaf inclination3 interacts with LIP1 to regulate rice leaf inclination through auxin signaling. PLoS Genet. 2018; 14(11):e1007829. PMC: 6289470. DOI: 10.1371/journal.pgen.1007829. View

Doebley J, Stec A, Gustus C . teosinte branched1 and the origin of maize: evidence for epistasis and the evolution of dominance. Genetics. 1995; 141(1):333-46. PMC: 1206731. DOI: 10.1093/genetics/141.1.333. View

Dong H, Zhao H, Xie W, Han Z, Li G, Yao W . A Novel Tiller Angle Gene, TAC3, together with TAC1 and D2 Largely Determine the Natural Variation of Tiller Angle in Rice Cultivars. PLoS Genet. 2016; 12(11):e1006412. PMC: 5096673. DOI: 10.1371/journal.pgen.1006412. View

He J, Shao G, Wei X, Huang F, Sheng Z, Tang S . Fine mapping and candidate gene analysis of qTAC8, a major quantitative trait locus controlling tiller angle in rice (Oryza sativa L.). PLoS One. 2017; 12(5):e0178177. PMC: 5444791. DOI: 10.1371/journal.pone.0178177. View

Huang L, Wang W, Zhang N, Cai Y, Liang Y, Meng X . LAZY2 controls rice tiller angle through regulating starch biosynthesis in gravity-sensing cells. New Phytol. 2021; 231(3):1073-1087. DOI: 10.1111/nph.17426. View

Jin J, Huang W, Gao J, Yang J, Shi M, Zhu M . Genetic control of rice plant architecture under domestication. Nat Genet. 2008; 40(11):1365-9. DOI: 10.1038/ng.247. View

Kanehisa M, Goto S . KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 1999; 28(1):27-30. PMC: 102409. DOI: 10.1093/nar/28.1.27. View

Kothari K, Dansana P, Giri J, Tyagi A . Rice Stress Associated Protein 1 (OsSAP1) Interacts with Aminotransferase (OsAMTR1) and Pathogenesis-Related 1a Protein (OsSCP) and Regulates Abiotic Stress Responses. Front Plant Sci. 2016; 7:1057. PMC: 4949214. DOI: 10.3389/fpls.2016.01057. View

10.

Lavy M, Estelle M . Mechanisms of auxin signaling. Development. 2016; 143(18):3226-9. PMC: 5047657. DOI: 10.1242/dev.131870. View

11.

Lee Y, Kende H . Expression of alpha-expansin and expansin-like genes in deepwater rice. Plant Physiol. 2002; 130(3):1396-405. PMC: 166658. DOI: 10.1104/pp.008888. View

12.

Li P, Wang Y, Qian Q, Fu Z, Wang M, Zeng D . LAZY1 controls rice shoot gravitropism through regulating polar auxin transport. Cell Res. 2007; 17(5):402-10. DOI: 10.1038/cr.2007.38. View

13.

Li H, Sun H, Jiang J, Sun X, Tan L, Sun C . TAC4 controls tiller angle by regulating the endogenous auxin content and distribution in rice. Plant Biotechnol J. 2020; 19(1):64-73. PMC: 7769243. DOI: 10.1111/pbi.13440. View

14.

Lin R, Wang H . Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport. Plant Physiol. 2005; 138(2):949-64. PMC: 1150410. DOI: 10.1104/pp.105.061572. View

15.

Liu Y, Wang H, Jiang Z, Wang W, Xu R, Wang Q . Genomic basis of geographical adaptation to soil nitrogen in rice. Nature. 2021; 590(7847):600-605. DOI: 10.1038/s41586-020-03091-w. View

16.

Liu H, Huang J, Zhang X, Liu G, Liang W, Zhu G . The RAC/ROP GTPase activator OsRopGEF10 functions in crown root development by regulating cytokinin signaling in rice. Plant Cell. 2022; 35(1):453-468. PMC: 9806555. DOI: 10.1093/plcell/koac297. View

17.

Lu C, Lin C, Lee K, Chen J, Huang L, Ho S . The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of rice. Plant Cell. 2007; 19(8):2484-99. PMC: 2002608. DOI: 10.1105/tpc.105.037887. View

18.

Lu G, Coneva V, Casaretto J, Ying S, Mahmood K, Liu F . OsPIN5b modulates rice (Oryza sativa) plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant J. 2015; 83(5):913-25. DOI: 10.1111/tpj.12939. View

19.

Tang F, Jiao B, Zhang M, He M, Su R, Luo K . , the R2R3 MYB transcription factor involved in secondary cell wall biosynthesis in poplar. Front Plant Sci. 2024; 14:1341245. PMC: 10828011. DOI: 10.3389/fpls.2023.1341245. View

20.

Uberti Manassero N, Viola I, Welchen E, Gonzalez D . TCP transcription factors: architectures of plant form. Biomol Concepts. 2014; 4(2):111-27. DOI: 10.1515/bmc-2012-0051. View