A Combination of Gold Nanoparticles and Laser Photobiomodulation to Boost Antioxidant Defenses in the Recovery of Muscle Injuries Caused by Bothrops Jararaca Venom

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2025 Jan 29

PMID 39878875

Authors

Mirian Ivens Fagundes

Nathalia Coral Galvani

Camille M Generoso

Ellen De-Pieri

Rahisa Scussel

Bruna B Fernandes

Igor Ramos Lima

Jessica S Abel

Maria Eduarda A B Correa

Jonathann C Possato

Paulo E Feuser

Thiago A M de Andrade

Carlos Chavez-Olortegui

Paulo Cesar Lock Silveira

Ricardo Andrez Machado-de-Avila

Affiliations

Soon will be listed here.

Abstract

Methods: 108 Swiss male mice were used, divided into nine groups (n = 12) with different combinations of treatments. Animals were inoculated with 250 µg of B. jararaca venom at the right gastrocnemius muscle. The treatment started 12 h after the venom injection and occurred daily for 7 days. AS was administered as recommended by the Ministry of Health. Low-power aluminum gallium indium phosphide (AlGaInP) with continuous emission (wavelength 660 nm; average power 30 mW ± 20%; beam size of 0.06310 cm; and dose of 2 J for ± 1 min per point). Irradiation was applied to five distinct areas surrounding the wound, at a perpendicular angle to the skin. The groups treated with GNPs received treatment through the application of 1 mL of 20 nm GNPs (30 mg/L). Muscle, heart, and kidneys were removed for histological and biochemical analysis.

Results: GNPs can improve the results achieved by PBM and the standard treatment, presenting significant benefits in histological parameters, such as the increase of new blood vessels, fibroblasts, collagen, and a reduction of inflammatory infiltrate.

Conclusion: GNPs can be considered an option to accelerate the recovery of Bothrops lesions.

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