Two NADPH-dependent 2-ketogluconate Reductases Involved in 2-ketogluconate Assimilation in Sp. Strain CHM43

Overview

Journal Appl Environ Microbiol

Date 2025 Jan 29

PMID 39878490

Authors

Sakura Nakashima

Minenosuke Matsutani

Naoya Kataoka

Osao Adachi

Riku Yamashita

Kazunobu Matsushita

Uraiwan Tippayasak

Gunjana Theeragool

Toshiharu Yakushi

Affiliations

Soon will be listed here.

Abstract

Importance: 2-Keto-D-gluconate (2KG), a product of incomplete oxidation of glucose by acetic acid bacteria including spp., is used for various purposes, including in the food industry. also consumes 2KG via an unclear metabolic pathway. It is reported that spp. and phosphorylate 2KG in the first step of 2KG metabolism, but some enteric bacteria including reduce 2KG. This study evaluated the 2KG consumption ability of a mutant derivative of a strain of that lacks two putative 2KGR-encoding genes. The mutant strain did not consume 2KG at all; the two 2KGRs were each found to catalyze 2KG reduction. It is concluded that reduction of 2KG is the committed step in 2KG metabolism in . The results presented here give insights that might facilitate improvement of 2KG production systems that use .

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