Identification of a Novel TOP2B::AFF2 Fusion Gene in B-cell Acute Lymphoblastic Leukemia

Overview

Journal Sci Rep

Date 2025 Jan 25

PMID 39863655

Authors

Tao Liu

Yang Wang

Xi-Zhou An

Jiaqi Liu

Yuqin Wu

Yan Xiang

Yong-Jie Zhang

Lan Huang

Jia-Cheng Li

Yu-Zhuo-Pu Li

Jie Yu

Affiliations

Soon will be listed here.

Abstract

Genetic alterations play a pivotal role in leukemic clonal transformation, significantly influencing disease pathogenesis and clinical outcomes. Here, we report a novel fusion gene and investigate its pathogenic role in acute lymphoblastic leukemia (ALL). We engineer a transposon transfection system expressing the TOP2B::AFF2 transcript and introduce it into Ba/F3 cells. Functional studies, including proliferation, cell cycle, and apoptosis assays, were conducted to assess the fusion gene's impact. In vitro assays reveal that the TOP2B::AFF2 fusion significantly enhances Ba/F3 cell proliferation and G1/S phase transition while suppressing differentiation and apoptosis. This study identifies TOP2B::AFF2 as a potential oncogenic driver. However, further validation through in vivo studies are warranted to fully elucidate the fusion gene's role in leukemogenesis.

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