The Role of Naphthaleneacetic Acid and 1-Methylcyclopropene in Preventing Preharvest Berry Dropping in L

Overview

Journal Plants (Basel)

Date 2025 Jan 25

PMID 39861632

Authors

Antonio Carlomagno

Claudio Bonghi

Giuseppe Montanaro

Alessandra Ferrandino

Angela Rasori

Vitale Nuzzo

Vittorino Novello

Affiliations

Soon will be listed here.

Abstract

Fruit dropping represents a concern in many fruit species, including L. This research investigated the role of two plant growth regulators (PGRs), naphthaleneacetic acid (NAA) and 1-methylcyclopropene (1-MCP), in mitigating preharvest berry dropping (PHBD) through affecting ethylene (ET) and auxin (AUX) metabolism and interactions, key hormones involved in abscission. The experiment was carried out on cv. Dolcetto, with PGR treatments applied at 43, 53, and 90 days after anthesis (DAA) for NAA and at 56 DAA for 1-MCP. Berry dropping incidence, yield parameters, and transcript levels of genes related to ET and AUX pathways were analyzed, including , , , , , and . Both NAA and 1-MCP significantly reduced PHBD, with NAA achieving a 92% reduction and 1-MCP an 82% reduction compared to control vines. Transcript analysis revealed differential gene expression patterns, indicating that NAA affects the ET biosynthesis pathway, while 1-MCP interferes with ET receptor signaling. The results suggest that both PGRs effectively reduced berry dropping, providing a basis for integrated crop management strategies to mitigate PHBD in grapevine cultivars susceptible to this physiological disorder.

References

Simon P . Q-Gene: processing quantitative real-time RT-PCR data. Bioinformatics. 2003; 19(11):1439-40. DOI: 10.1093/bioinformatics/btg157. View

Wang K, Li H, Ecker J . Ethylene biosynthesis and signaling networks. Plant Cell. 2002; 14 Suppl:S131-51. PMC: 151252. DOI: 10.1105/tpc.001768. View

Ferrara G, Mazzeo A, Matarrese A, Pacucci C, Trani A, Fidelibus M . Ethephon As a Potential Abscission Agent for Table Grapes: Effects on Pre-Harvest Abscission, Fruit Quality, and Residue. Front Plant Sci. 2016; 7:620. PMC: 4885227. DOI: 10.3389/fpls.2016.00620. View

Bottcher C, Boss P, Davies C . Acyl substrate preferences of an IAA-amido synthetase account for variations in grape (Vitis vinifera L.) berry ripening caused by different auxinic compounds indicating the importance of auxin conjugation in plant development. J Exp Bot. 2011; 62(12):4267-80. PMC: 3153680. DOI: 10.1093/jxb/err134. View

Olsson V, Butenko M . Abscission in plants. Curr Biol. 2018; 28(8):R338-R339. DOI: 10.1016/j.cub.2018.02.069. View

Ziliotto F, Corso M, Rizzini F, Rasori A, Botton A, Bonghi C . Grape berry ripening delay induced by a pre-véraison NAA treatment is paralleled by a shift in the expression pattern of auxin- and ethylene-related genes. BMC Plant Biol. 2012; 12:185. PMC: 3564861. DOI: 10.1186/1471-2229-12-185. View

Merelo P, Agusti J, Arbona V, Costa M, Estornell L, Gomez-Cadenas A . Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus. Front Plant Sci. 2017; 8:126. PMC: 5296326. DOI: 10.3389/fpls.2017.00126. View

Botton A, Girardi F, Ruperti B, Brilli M, Tijero V, Eccher G . Grape Berry Responses to Sequential Flooding and Heatwave Events: A Physiological, Transcriptional, and Metabolic Overview. Plants (Basel). 2022; 11(24). PMC: 9788187. DOI: 10.3390/plants11243574. View

Garcia-Rojas M, Meneses M, Oviedo K, Carrasco C, Defilippi B, Gonzalez-Aguero M . Exogenous gibberellic acid application induces the overexpression of key genes for pedicel lignification and an increase in berry drop in table grape. Plant Physiol Biochem. 2018; 126:32-38. DOI: 10.1016/j.plaphy.2018.02.009. View

10.

Binder B . Ethylene signaling in plants. J Biol Chem. 2020; 295(22):7710-7725. PMC: 7261785. DOI: 10.1074/jbc.REV120.010854. View

11.

Wang P, Yu A, Ji X, Mu Q, Haider M, Wei R . Transcriptome and metabolite integrated analysis reveals that exogenous ethylene controls berry ripening processes in grapevine. Food Res Int. 2022; 155:111084. DOI: 10.1016/j.foodres.2022.111084. View

12.

Zanchin A, Marcato C, Trainotti L, Casadoro G, Rascio N . Characterization of abscission zones in the flowers and fruits of peach [Prunus persica (L.) Batsch]. New Phytol. 2021; 129(2):345-354. DOI: 10.1111/j.1469-8137.1995.tb04305.x. View

13.

Tian Y, Xin W, Lin J, Ma J, He J, Wang X . Auxin Coordinates Achene and Receptacle Development During Fruit Initiation in . Front Plant Sci. 2022; 13:929831. PMC: 9301465. DOI: 10.3389/fpls.2022.929831. View

14.

Kou X, Feng Y, Yuan S, Zhao X, Wu C, Wang C . Different regulatory mechanisms of plant hormones in the ripening of climacteric and non-climacteric fruits: a review. Plant Mol Biol. 2021; 107(6):477-497. DOI: 10.1007/s11103-021-01199-9. View

15.

Clayton-Cuch D, Yu L, Shirley N, Bradley D, Bulone V, Bottcher C . Auxin Treatment Enhances Anthocyanin Production in the Non-Climacteric Sweet Cherry ( L.). Int J Mol Sci. 2021; 22(19). PMC: 8509301. DOI: 10.3390/ijms221910760. View

16.

Costantini E, Landi L, Silvestroni O, Pandolfini T, Spena A, Mezzetti B . Auxin synthesis-encoding transgene enhances grape fecundity. Plant Physiol. 2007; 143(4):1689-94. PMC: 1851826. DOI: 10.1104/pp.106.095232. View

17.

Zhang J, Ma Y, Dong C, Terry L, Watkins C, Yu Z . Meta-analysis of the effects of 1-methylcyclopropene (1-MCP) treatment on climacteric fruit ripening. Hortic Res. 2020; 7(1):208. PMC: 7713375. DOI: 10.1038/s41438-020-00405-x. View

18.

Botton A, Ruperti B . The Yes and No of the Ethylene Involvement in Abscission. Plants (Basel). 2019; 8(6). PMC: 6630578. DOI: 10.3390/plants8060187. View

19.

Castellarin S, Pfeiffer A, Sivilotti P, Degan M, Peterlunger E, Di Gaspero G . Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant Cell Environ. 2007; 30(11):1381-99. DOI: 10.1111/j.1365-3040.2007.01716.x. View

20.

Huang W, Tan C, Guo H . Ethylene in fruits: beyond ripening control. Hortic Res. 2024; 11(10):uhae229. PMC: 11480664. DOI: 10.1093/hr/uhae229. View