Genomic Insight into Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels

Overview

Journal Pathogens

Date 2025 Jan 25

PMID 39861013

Authors

Artun Yibar

Muhammed Duman

Hilal Ay

Nihed Ajmi

Gorkem Tasci

Fatma Gurler

Sabire Guler

Danny Morick

Izzet Burcin Saticioglu

Affiliations

Soon will be listed here.

Abstract

Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks. The results showed prevalence rates of 12.5% in FRMs and 4.2% in RTE-SMs, with as the most common species (46.7%). It was determined that the seasonal distribution of spp. prevalence in the samples was higher in the summer months. The genome sizes of the spp. ranged from approximately 3.9 to 6.1 Mb, with the GC contents varying between 41.9% and 50.4%. A total of 22 virulence factor (VF) classes and up to six antimicrobial resistance (AMR) genes were detected in different species. The presence of nine different biosynthetic gene clusters (BGCs), 27 prophage regions, and eight CRISPR/Cas systems in 15 strains provides information about their potential pathogenicity, survival strategies, and adaptation to different habitats. Overall, this study provides a comprehensive understanding of the genomic diversity of spp. isolated from FRM and RTE-SM samples, shedding light on the prevalence, pathogenicity, and toxicity mechanisms of -induced gastroenteritis.

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