Comparative Efficacy of Melatonin and Brassinolide in Mitigating the Adverse Effects of Cadmium on

Overview

Journal Int J Mol Sci

Publisher MDPI

Date 2025 Jan 25

PMID 39859406

Authors

Magdalena Chmur

Andrzej Bajguz

Affiliations

Soon will be listed here.

Abstract

Melatonin (MT) and brassinolide (BL) are phytohormones that regulate various physiological processes in plants. This study investigates their effects on when exposed to cadmium (Cd). Plant hormones were quantified using liquid chromatography-mass spectrometry, while photosynthetic pigments and phytochelatins (PCs) were analyzed through high-performance liquid chromatography. Protein, monosaccharide levels, and antioxidant activities were also spectrophotometrically measured. The findings reveal that MT and BL treatment decreased Cd accumulation in compared to plants only exposed to Cd. MT was particularly effective in reversing Cd-induced growth inhibition and reducing stress markers more significantly than BL. It also enhanced antioxidant activity and maintained higher levels of photosynthetic pigments, proteins, and sugars. Although BL was less effective in these aspects, it promoted greater synthesis of glutathione and PCs in Cd-exposed duckweed. Overall, both MT and BL alleviate the negative impact of Cd on , confirming their crucial role in supporting plant health under metal stress conditions.

References

Ha Y, Shang Y, Yang D, Nam K . Brassinosteroid reduces ABA accumulation leading to the inhibition of ABA-induced stomatal closure. Biochem Biophys Res Commun. 2018; 504(1):143-148. DOI: 10.1016/j.bbrc.2018.08.146. View

Bajguz A, Orczyk W, Golebiewska A, Chmur M, Piotrowska-Niczyporuk A . Occurrence of brassinosteroids and influence of 24-epibrassinolide with brassinazole on their content in the leaves and roots of Hordeum vulgare L. cv. Golden Promise. Planta. 2018; 249(1):123-137. DOI: 10.1007/s00425-018-03081-3. View

Wang M, Duan S, Zhou Z, Chen S, Wang D . Foliar spraying of melatonin confers cadmium tolerance in Nicotiana tabacum L. Ecotoxicol Environ Saf. 2018; 170:68-76. DOI: 10.1016/j.ecoenv.2018.11.127. View

Arnao M, Hernandez-Ruiz J . Melatonin as a regulatory hub of plant hormone levels and action in stress situations. Plant Biol (Stuttg). 2020; 23 Suppl 1:7-19. DOI: 10.1111/plb.13202. View

Appenroth K, Ziegler P, Sree K . Accumulation of starch in duckweeds (Lemnaceae), potential energy plants. Physiol Mol Biol Plants. 2021; 27(11):2621-2633. PMC: 8639912. DOI: 10.1007/s12298-021-01100-4. View

AEBI H . Catalase in vitro. Methods Enzymol. 1984; 105:121-6. DOI: 10.1016/s0076-6879(84)05016-3. View

Piotrowska-Niczyporuk A, Bonda-Ostaszewska E, Bajguz A . Mitigating Effect of -Zeatin on Cadmium Toxicity in . Cells. 2024; 13(8. PMC: 11049045. DOI: 10.3390/cells13080686. View

Amjadi Z, Namdjoyan S, Abolhasani Soorki A . Exogenous melatonin and salicylic acid alleviates cadmium toxicity in safflower (Carthamus tinctorius L.) seedlings. Ecotoxicology. 2021; 30(3):387-401. DOI: 10.1007/s10646-021-02364-y. View

Ahmad S, Kamran M, Zhou X, Ahmad I, Meng X, Javed T . Melatonin improves the seed filling rate and endogenous hormonal mechanism in grains of summer maize. Physiol Plant. 2020; 172(2):1059-1072. DOI: 10.1111/ppl.13282. View

10.

Zhang N, Sun Q, Zhang H, Cao Y, Weeda S, Ren S . Roles of melatonin in abiotic stress resistance in plants. J Exp Bot. 2014; 66(3):647-56. DOI: 10.1093/jxb/eru336. View

11.

Gu Q, Chen Z, Yu X, Cui W, Pan J, Zhao G . Melatonin confers plant tolerance against cadmium stress via the decrease of cadmium accumulation and reestablishment of microRNA-mediated redox homeostasis. Plant Sci. 2017; 261:28-37. DOI: 10.1016/j.plantsci.2017.05.001. View

12.

Malejko J, Deoniziak K, Tomczuk M, Dlugokencka J, Godlewska-Zylkiewicz B . Puparial Cases as Toxicological Indicators: Bioaccumulation of Cadmium and Thallium in the Forensically Important Blowfly . Front Chem. 2020; 8:586067. PMC: 7716273. DOI: 10.3389/fchem.2020.586067. View

13.

Honig M, Plihalova L, Husickova A, Nisler J, Dolezal K . Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis. Int J Mol Sci. 2018; 19(12). PMC: 6321018. DOI: 10.3390/ijms19124045. View

14.

Beauchamp C, Fridovich I . Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44(1):276-87. DOI: 10.1016/0003-2697(71)90370-8. View

15.

Hoque M, Tahjib-Ul-Arif M, Hannan A, Sultana N, Akhter S, Hasanuzzaman M . Melatonin Modulates Plant Tolerance to Heavy Metal Stress: Morphological Responses to Molecular Mechanisms. Int J Mol Sci. 2021; 22(21). PMC: 8584185. DOI: 10.3390/ijms222111445. View

16.

Olds C, Glennon E, Luckhart S . Abscisic acid: new perspectives on an ancient universal stress signaling molecule. Microbes Infect. 2018; 20(9-10):484-492. DOI: 10.1016/j.micinf.2018.01.009. View

17.

Schaedle M . Chloroplast glutathione reductase. Plant Physiol. 1977; 59(5):1011-2. PMC: 543356. DOI: 10.1104/pp.59.5.1011. View

18.

Simura J, Antoniadi I, Siroka J, Tarkowska D, Strnad M, Ljung K . Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics. Plant Physiol. 2018; 177(2):476-489. PMC: 6001343. DOI: 10.1104/pp.18.00293. View

19.

Tousi S, Zoufan P, Ghahfarrokhie A . Alleviation of cadmium-induced phytotoxicity and growth improvement by exogenous melatonin pretreatment in mallow (Malva parviflora) plants. Ecotoxicol Environ Saf. 2020; 206:111403. DOI: 10.1016/j.ecoenv.2020.111403. View

20.

Niu K, Zhu R, Wang Y, Zhao C, Ma H . 24-epibrassinolide improves cadmium tolerance and lateral root growth associated with regulating endogenous auxin and ethylene in Kentucky bluegrass. Ecotoxicol Environ Saf. 2024; 249():114460. DOI: 10.1016/j.ecoenv.2022.114460. View