The AP2/ERF Transcription Factor ERF56 Negatively Regulating Nitrate-Dependent Plant Growth in

Overview

Journal Int J Mol Sci

Publisher MDPI

Date 2025 Jan 25

PMID 39859331

Authors

Guoqi Yao

Chunhua Mu

Zhenwei Yan

Shijun Ma

Xia Liu

Yue Sun

Jing Hou

Qiantong Liu

Bing Cao

Juan Shan

Bingying Leng

Affiliations

Soon will be listed here.

Abstract

ERF56, a member of the APETALA2/ETHYLENE-RESPONSIVE FACTOR (AP2/ERF) transcription factor (TF) family, was reported to be an early nitrate-responsive TF in . But the function of in nitrate signaling remains not entirely clear. This study aimed to investigate the role of in nitrate-dependent plant growth and nitrate signaling. We confirmed with reverse transcription quantitative PCR (RT-qPCR) that the transcription of is quickly induced by nitrate. overexpressors displayed decreased nitrate-dependent plant growth, while mutants exhibited increased plant growth. Confocal imaging demonstrated that ERF56 is localized into nuclei. Assays with the glucuronidase (GUS) reporter showed that is mainly expressed at the region of maturation of roots and in anthers. The dual-luciferase assay manifested that the transcription of is not directly regulated by NIN-LIKE PROTEIN 7 (NLP7). The transcriptome analysis identified 1038 candidate genes regulated by ERF56 directly. A gene ontology (GO) over-representation analysis showed that is involved in the processes of water transport, inorganic molecule transmembrane transport, secondary metabolite biosynthesis, and cell wall organization. We revealed that represses nitrate-dependent growth through regulating the processes of inorganic molecule transmembrane transport, the secondary metabolite biosynthesis, and cell wall organization.

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