Functional Characterization of OsCSN1 in the Agronomic Trait Control of Rice Seedlings Under Far-Red Light

Overview

Journal Int J Mol Sci

Publisher MDPI

Date 2025 Jan 25

PMID 39859235

Authors

Yanxi Liu

Hua Zeng

Yuqing Shang

Hexin Zhang

Tongtong Jiao

Le Yin

Jinyuan Yang

Miao Xu

Jingmei Lu

Ming Wu

Liquan Guo

Affiliations

Soon will be listed here.

Abstract

The COP9 signalosome (CSN) is a highly conserved multi-subunit protein complex, with CSN1 being its largest and most conserved subunit. The N-terminal function of CSN1 plays a pivotal and intricate role in plant photomorphogenesis and seedling development. Moreover, CSN is essential for far-red light-mediated photomorphogenesis in seedlings, but the function of OsCSN1 in seedling growth and development under far-red light conditions has not been determined. This study investigates the function of OsCSN1 under far-red light through phenotypic analysis of wild type and mutant seedlings. Additionally, the effect of the N-terminal region of OsCSN1 on rice seedling growth and development was examined. The addition of exogenous hormone gibberellin (GA) and gibberellin synthesis inhibitor paclobutrazol (PAC) resulted in notable changes in phenotypes and the expression of key proteins, including CUL4 and SLR1. The findings indicate that OsCSN1 functions as a positive regulator of plant height under far-red light and inhibits root elongation. Under far-red light, OsCSN1 integrates into the COP9 complex and regulates the nuclear localization of COP1. Through its interaction with CUL4 in the CULLIN-RING family, OsCSN1 facilitates the ubiquitin-mediated degradation of SLR1, thereby influencing the growth of rice seedlings. The regulatory function of OsCSN1 in seedling growth and development under far-red light predominantly relies on the 32 amino acids of its N-terminal region. The results of this study can provide new ideas for rice breeding and genetic improvement. Based on the study of key regulatory factors such as OsCSN1, new varieties that can make better use of far-red light signals can be cultivated to enhance crop adaptability and productivity.

References

Kotiguda G, Weinberg D, Dessau M, Salvi C, Serino G, Chamovitz D . The organization of a CSN5-containing subcomplex of the COP9 signalosome. J Biol Chem. 2012; 287(50):42031-41. PMC: 3516749. DOI: 10.1074/jbc.M112.387977. View

Wang J, Chen C, Chien C, Hsieh H . FAR-RED INSENSITIVE219 modulates CONSTITUTIVE PHOTOMORPHOGENIC1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiol. 2011; 156(2):631-46. PMC: 3177264. DOI: 10.1104/pp.111.177667. View

Clough R, Lohman K, Marita J, Walker J, Gatz C, Vierstra R . Sequences within both the N- and C-terminal domains of phytochrome A are required for PFR ubiquitination and degradation. Plant J. 1999; 17(2):155-67. DOI: 10.1046/j.1365-313x.1999.00360.x. View

Sharrock R, Clack T . Heterodimerization of type II phytochromes in Arabidopsis. Proc Natl Acad Sci U S A. 2004; 101(31):11500-5. PMC: 509229. DOI: 10.1073/pnas.0404286101. View

Jin D, Wu M, Li B, Bucker B, Keil P, Zhang S . The COP9 Signalosome regulates seed germination by facilitating protein degradation of RGL2 and ABI5. PLoS Genet. 2018; 14(2):e1007237. PMC: 5834205. DOI: 10.1371/journal.pgen.1007237. View

Mahmood T, Yang P . Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012; 4(9):429-34. PMC: 3456489. DOI: 10.4103/1947-2714.100998. View

Hofmann K, Bucher P . The PCI domain: a common theme in three multiprotein complexes. Trends Biochem Sci. 1998; 23(6):204-5. DOI: 10.1016/s0968-0004(98)01217-1. View

Bae G, Choi G . Decoding of light signals by plant phytochromes and their interacting proteins. Annu Rev Plant Biol. 2008; 59:281-311. DOI: 10.1146/annurev.arplant.59.032607.092859. View

Chen H, Huang X, Gusmaroli G, Terzaghi W, Lau O, Yanagawa Y . Arabidopsis CULLIN4-damaged DNA binding protein 1 interacts with CONSTITUTIVELY PHOTOMORPHOGENIC1-SUPPRESSOR OF PHYA complexes to regulate photomorphogenesis and flowering time. Plant Cell. 2010; 22(1):108-23. PMC: 2828697. DOI: 10.1105/tpc.109.065490. View

10.

Takano M, Inagaki N, Xie X, Kiyota S, Baba-Kasai A, Tanabata T . Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice. Proc Natl Acad Sci U S A. 2009; 106(34):14705-10. PMC: 2732857. DOI: 10.1073/pnas.0907378106. View

11.

Bao S, Hua C, Shen L, Yu H . New insights into gibberellin signaling in regulating flowering in Arabidopsis. J Integr Plant Biol. 2019; 62(1):118-131. DOI: 10.1111/jipb.12892. View

12.

Lee K, Lopez-Molina L . Control of seed germination in the shade. Cell Cycle. 2012; 11(24):4489-90. PMC: 3562285. DOI: 10.4161/cc.22667. View

13.

Shimizu H, Tanabata T, Xie X, Inagaki N, Takano M, Shinomura T . Phytochrome-mediated growth inhibition of seminal roots in rice seedlings. Physiol Plant. 2009; 137(3):289-97. DOI: 10.1111/j.1399-3054.2009.01277.x. View

14.

Wei N, Deng X . COP9: a new genetic locus involved in light-regulated development and gene expression in arabidopsis. Plant Cell. 1992; 4(12):1507-18. PMC: 160237. DOI: 10.1105/tpc.4.12.1507. View

15.

Sheerin D, Menon C, Zur Oven-Krockhaus S, Enderle B, Zhu L, Johnen P . Light-activated phytochrome A and B interact with members of the SPA family to promote photomorphogenesis in Arabidopsis by reorganizing the COP1/SPA complex. Plant Cell. 2015; 27(1):189-201. PMC: 4330587. DOI: 10.1105/tpc.114.134775. View

16.

Hoecker U, Xu Y, Quail P . SPA1: a new genetic locus involved in phytochrome A-specific signal transduction. Plant Cell. 1998; 10(1):19-33. PMC: 143933. DOI: 10.1105/tpc.10.1.19. View

17.

Deng X, Matsui M, Wei N, Wagner D, Chu A, Feldmann K . COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain. Cell. 1992; 71(5):791-801. DOI: 10.1016/0092-8674(92)90555-q. View

18.

Schwechheimer C, Deng X . COP9 signalosome revisited: a novel mediator of protein degradation. Trends Cell Biol. 2001; 11(10):420-6. DOI: 10.1016/s0962-8924(01)02091-8. View

19.

Wang X, Kang D, Feng S, Serino G, Schwechheimer C, Wei N . CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome. Mol Biol Cell. 2002; 13(2):646-55. PMC: 65656. DOI: 10.1091/mbc.01-08-0427. View

20.

Chen S, Wang X, Tan G, Zhou W, Wang G . Gibberellin and the plant growth retardant Paclobutrazol altered fruit shape and ripening in tomato. Protoplasma. 2019; 257(3):853-861. DOI: 10.1007/s00709-019-01471-2. View