Comparison and Classification of LMW-GS Genes at Loci of Common Wheat

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2025 Jan 25

PMID 39858637

Authors

Yongying Zhao

Xianlin Zhao

Zhiguo Xiang

Dan Zhang

Hongshan Yang

Affiliations

Soon will be listed here.

Abstract

Background: The low molecular weight glutenin subunits (LMW-GS) of wheat have great effects on food processing quality, but the resolution of LMW-GS and the scoring of their alleles by direct analysis of proteins are difficult due to the larger number of expressed subunits and high similarity of DNA sequences. It is important to identify and classify the LMW-GS genes in order to recognize the LMW-GS alleles clearly and develop the functional markers.

Methods: The LMW-GS genes registered in GenBank were searched at NCBI, and 593 genes with complete coding sequences were obtained, including 146 , 136 , and 311 . Sequence analysis and characterization of DNA and deduced amino acids were performed using the software DNAman.

Results: The alignment and classification showed that there were at least 9 genes with 69 allelic variants at the locus, 11 genes with 64 allelic variants at the locus, and 10 genes with 96 variants at the locus, respectively. Furthermore, the specificity of some genes and their variations was analyzed.

Conclusions: The results were beneficial to understanding the LMW-GS genes fully and to developing the functional markers and will provide a theoretical reference for the quality improvement of wheat variety.

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