Suppression of Nodule Formation by RNAi Knock-Down of in

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2025 Jan 25

PMID 39858605

Authors

Fuxiao Jin

Danxia Ke

Lu Lu

Qianqian Hu

Chanjuan Zhang

Chao Li

Wanwan Liang

Songli Yuan

Haifeng Chen

Affiliations

Soon will be listed here.

Abstract

Background/objectives: The balanced regulation of innate immunity plays essential roles in rhizobial infection and the establishment and maintenance of symbiosis. The evolutionarily conserved cell death suppressor Bax inhibitor-1 plays dual roles in nodule symbiosis, providing a valuable clue in balancing immunity and symbiosis, while it remains largely unexplored in the legume .

Methods/results: In the present report, the gene family of was identified and characterized. We identified 6 genes that translate into peptides containing 240-255 amino acids with different structural characteristics and isoelectric points. We performed phylogenetic analyses and detected evolutionary conservation and divergence among BI-1 proteins from , , , , and . Expression profiles among different roots indicated that the inoculation of MAFF303099 significantly increased the expression of most of the BI-1 family genes. We down-regulated the transcripts of by RNA interference and observed that promotes nodulation and nodule formation.

Conclusions: These discoveries shed light on the functions of genes in , and simultaneously emphasize the potential application of in enhancing the symbiotic nitrogen fixation ability of legumes.

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