Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms

Overview

Journal Animals (Basel)

Date 2025 Jan 25

PMID 39858273

Authors

Chaitawat Sirisereewan

Thanh Che Nguyen

Nanthiya Iampraphat

Hongyao Lin

Leonardo Ellerma

Pisit Sirithanyakul

Roongtham Kedkovid

Roongroje Thanawongnuwech

Affiliations

Soon will be listed here.

Abstract

Porcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. Vaccination is a key strategy for PEDV control, but optimizing regimens based on herd status is essential for improving immunity and protection. This study evaluated immune responses to different vaccination protocols using a PED replicon vaccine (PED-RP) in Thai swine farms with varying PED statuses. Two field trials were conducted: Experiment 1 ( = 120) on a PED-negative farm used a killed vaccine followed by PED-RP, while Experiment 2 ( = 42) on a PED-positive farm used a modified-live vaccine (MLV) followed by PED-RP. In Experiment 1, gilts boosted with PED-RP during gestation showed significantly higher colostrum IgA levels compared to those receiving two doses of the killed vaccine. In Experiment 2, priming with MLV followed by PED-RP resulted in increased neutralizing antibody titers and seropositivity, outperforming the MLV-only regimen after acclimatization. These findings suggest that using PED-RP as a booster can enhance serum and lactogenic immunity, providing a promising strategy for improving immune responses in both PED-negative and PED-positive herds. The study highlights the importance of tailored vaccination strategies to optimize herd immunity and protect piglets from PEDV.

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