Enhancement of Growth, Antioxidant Activity, and Immunity in Nile Tilapia () Through Recombinant Expressing L-Gulonolactone Oxidase

Overview

Journal Antioxidants (Basel)

Date 2025 Jan 25

PMID 39857384

Authors

Jirawadee Kaewda

Surintorn Boonanuntanasarn

Papungkorn Sangsawad

Pimpisut Manassila

Chatsirin Nakharuthai

Affiliations

Soon will be listed here.

Abstract

Due to its lack of the L-gulonolactone oxidase () enzyme, Nile tilapia is unable to synthesize vitamin C; thus, it requires an adequate level of exogenous vitamin C in its diet. To enhance antioxidant properties and vitamin C-related effects, we employed recombinant technology to integrate the -encoding gene into the chromosome. In this study, fish were divided into four groups: those fed with a basal diet (CON), a basal diet + vitamin C (VC), a basal diet + wild-type (BS), and a basal diet + recombinant (BS+GULO). After 90 days of the feeding trial, the BS+GULO groups showed the highest improvements in final weight, weight gain, specific growth rate, average daily gain, and relative growth rate. The VC, BS, and BS+GULO groups exhibited increased total immunoglobulin and lysozyme activity; however, only the VC and BS+GULO groups showed elevated alternative complement 50 levels, phagocytic activity and improved antioxidant parameters compared to the control. HPLC and qRT-PCR analyses revealed elevated serum vitamin C and intestinal mRNA levels in the BS+GULO group. A challenge test showed increased pro-inflammatory gene expression and immune response against in the BS+GULO group, indicating improved antagonistic activity over wild-type .

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