Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, As Well As Anti-Alkaline Regulation Via in the Cerebral Ganglion of Chinese Mitten Crab Under Alkaline Stress

Overview

Journal Biology (Basel)

Publisher MDPI

Date 2025 Jan 25

PMID 39857314

Authors

Meiyao Wang

Jun Zhou

Jiachun Ge

Gangchun Xu

Yongkai Tang

Affiliations

Soon will be listed here.

Abstract

(1) Background: Global climate change is intensifying, and the vigorous development and utilization of saline-alkali land is of great significance. As an important economic aquatic species in the context of saline-alkali aquaculture, it is highly significant to explore the regulatory mechanisms of under alkaline conditions. In particular, the brain (cerebral ganglion for crustaceans) serves as a vital regulatory organ in response to environmental stress; (2) Methods: In this study, a comparative transcriptome approach was employed to investigate the key regulatory genes and molecular regulatory mechanisms in the cerebral ganglion of under alkaline stress. (3) Results: The results demonstrated that the cerebral ganglion of exhibited a positive response to acute alkaline stress. Pathways associated with signal transduction and substance transportation, such as "phagosome" and "regulation of actin cytoskeleton", along with regulatory genes involved in antioxidation, were upregulated synergistically to maintain homeostasis under alkaline stress. Furthermore, it was discovered for the first time that plays a positive regulatory role in the adaptation of to alkalinity. (4) Conclusions: The present study elucidates the molecular regulatory pattern of the cerebral ganglion in under acute alkaline stress as well as revealing a novel role of in facilitating adaptation to alkalinity in , providing valuable theoretical insights into the molecular regulatory mechanisms underlying the responses of cerebral ganglia to saline-alkali environments. These findings also offer a theoretical reference for promoting the sustainable development of the breeding industry under saline-alkali conditions.

References

Xie Z, Zhao M, Yan C, Kong W, Lan F, Narengaowa . Cathepsin B in programmed cell death machinery: mechanisms of execution and regulatory pathways. Cell Death Dis. 2023; 14(4):255. PMC: 10082344. DOI: 10.1038/s41419-023-05786-0. View

Loy T, Van Hiel M, Vandersmissen H, Poels J, Mendive F, Vassart G . Evolutionary conservation of bursicon in the animal kingdom. Gen Comp Endocrinol. 2007; 153(1-3):59-63. DOI: 10.1016/j.ygcen.2006.12.004. View

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

Estrada-Cardenas P, Peregrino-Uriarte A, Yepiz-Plascencia G . Glutathione peroxidase 4 knock-down triggers ferroptosis in Penaeus vannamei hepatopancreas during hypoxia and reoxygenation. Fish Shellfish Immunol. 2024; :109201. DOI: 10.1016/j.fsi.2023.109201. View

Lawrence R, Zoncu R . The lysosome as a cellular centre for signalling, metabolism and quality control. Nat Cell Biol. 2019; 21(2):133-142. DOI: 10.1038/s41556-018-0244-7. View

Dewey E, McNabb S, Ewer J, Kuo G, Takanishi C, Truman J . Identification of the gene encoding bursicon, an insect neuropeptide responsible for cuticle sclerotization and wing spreading. Curr Biol. 2004; 14(13):1208-13. DOI: 10.1016/j.cub.2004.06.051. View

Wang J, Sun L, Li X, Tao S, Wang F, Shi Y . Alkali exposure induces autophagy through activation of the MAPKpathway by ROS and inhibition of mTOR in Eriocheir sinensis. Aquat Toxicol. 2023; 258:106481. DOI: 10.1016/j.aquatox.2023.106481. View

Kaziro Y, Itoh H, Kozasa T, Nakafuku M, Satoh T . Structure and function of signal-transducing GTP-binding proteins. Annu Rev Biochem. 1991; 60:349-400. DOI: 10.1146/annurev.bi.60.070191.002025. View

Jiang X, Liu C, Hu Y, Shao K, Tang X, Zhang L . Climate-induced salinization may lead to increased lake nitrogen retention. Water Res. 2022; 228(Pt A):119354. DOI: 10.1016/j.watres.2022.119354. View

10.

Xu F, Xing X, Zhang K, Zhang C . Status and prospects of product processing and sustainable utilization of Chinese mitten crab (). Heliyon. 2024; 10(12):e32922. PMC: 11238004. DOI: 10.1016/j.heliyon.2024.e32922. View

11.

Xu J, Li Q, Xu L, Wang S, Jiang Y, Zhao Z . Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lake. BMC Genomics. 2013; 14:682. PMC: 3852516. DOI: 10.1186/1471-2164-14-682. View

12.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

13.

Zhang R, Zhao Z, Li M, Luo L, Wang S, Guo K . Metabolomics analysis reveals the response mechanism to carbonate alkalinity toxicity in the gills of Eriocheir sinensis. Comp Biochem Physiol C Toxicol Pharmacol. 2022; 263:109487. DOI: 10.1016/j.cbpc.2022.109487. View

14.

Fu C, Li F, Wang L, Wang A, Yu J, Wang H . Comparative transcriptology reveals effects of circadian rhythm in the nervous system on precocious puberty of the female Chinese mitten crab. Comp Biochem Physiol Part D Genomics Proteomics. 2018; 29:67-73. DOI: 10.1016/j.cbd.2018.11.004. View

15.

Webster S, Wilcockson D, Mrinalini , Sharp J . Bursicon and neuropeptide cascades during the ecdysis program of the shore crab, Carcinus maenas. Gen Comp Endocrinol. 2012; 182:54-64. DOI: 10.1016/j.ygcen.2012.11.018. View

16.

Chung J, Katayama H, Dircksen H . New functions of arthropod bursicon: inducing deposition and thickening of new cuticle and hemocyte granulation in the blue crab, Callinectes sapidus. PLoS One. 2012; 7(9):e46299. PMC: 3460823. DOI: 10.1371/journal.pone.0046299. View

17.

Jin S, Zhou R, Gao X, Xiong Y, Zhang W, Qiao H . Identification of the effects of alkalinity exposure on the gills of oriental river prawns, Macrobrachium nipponense. BMC Genomics. 2024; 25(1):765. PMC: 11304644. DOI: 10.1186/s12864-024-10659-7. View

18.

van der Ploeg R, Cramwinckel M, Kocken I, Leutert T, Bohaty S, Fokkema C . North Atlantic surface ocean warming and salinization in response to middle Eocene greenhouse warming. Sci Adv. 2023; 9(4):eabq0110. PMC: 9876553. DOI: 10.1126/sciadv.abq0110. View

19.

Chen H, Feng Y, Cui J, Wang X . Response of CRH system in brain and gill of marine medaka to seawater acidification. Fish Physiol Biochem. 2024; 50(3):1225-1236. DOI: 10.1007/s10695-024-01332-7. View

20.

Wang M, Tang Y, Yu J, Su S, Li J, Yu F . Molecular insights into the sex-differential regulation of signal transduction in the cerebral ganglion and metabolism in the hepatopancreas of Eriocheir sinensis during reproduction. Genomics. 2019; 112(1):71-81. DOI: 10.1016/j.ygeno.2019.10.014. View