Oral Administration of Recombinant Lactococcus Lactis Co-expressing Fusion VP1 Protein of Duck Hepatitis a Virus Type 1 and 3 Protects Ducklings Against Infection

Overview

Journal Poult Sci

Publisher Elsevier

Date 2025 Jan 24

PMID 39854966

Authors

Xiaoting Zhang

Hui Yan

Lei Bei

Guige Xu

Mingrui Zhao

Ruihua Zhang

Yu Meng

Yanli Zhu

Zhijing Xie

Shijin Jiang

Affiliations

Soon will be listed here.

Abstract

Duck viral hepatitis (DVH) is one of the most common diseases of waterfowl. Duck hepatitis A virus type 1 (DHAV-1) and type 3 (DHAV-3) have been on the rise seriously endangering the development of duck farming. In this study, we constructed a recombinant Lactococcus lactis (L. lactis) co-expressing the fusion VP1 protein of DHAV-1 and DHAV-3. Western blot and immunofluorescence analyses confirmed that the fusion VP1 protein was expressed on the cell surface of recombinant L. lactis stain. After immunization with the recombinant L. lactis by oral administration, the ducklings were challenged with highly pathogenic DHAV-1 and DHAV-3 by natural infection. The results showed that the recombinant L. lactis induced the specific IgG antibodies, and significantly increased the level of mucosal secretory immunoglobulin A (sIgA) and cytokines, such as interleukin-4 (IL-4), interleukin-10 (IL-10), and interferon gamma (IFN-γ). The immunized ducklings delayed death for around 4 days, and reduced mortality with the relative percent survival (RPS) being 64.71 %, 61.54 % and 57.89 % in DHAV-1, DHAV-3 and DHAV-1 + 3 infection group respectively. These results showed that the recombinant L. lactis constructed in this study provides a promising candidate for prevention and control of DHAV-1 and DHAV-3 infection.

References

Avall-Jaaskelainen S, Palva A . Secretion of biologically active porcine interleukin-2 by Lactococcus lactis. Vet Microbiol. 2006; 115(1-3):278-83. DOI: 10.1016/j.vetmic.2006.02.007. View

Szatraj K, Szczepankowska A, Chmielewska-Jeznach M . Lactic acid bacteria - promising vaccine vectors: possibilities, limitations, doubts. J Appl Microbiol. 2017; 123(2):325-339. PMC: 7166332. DOI: 10.1111/jam.13446. View

Chen S, Liu C, Hu S . Dietary administration of probiotic Paenibacillus ehimensis NPUST1 with bacteriocin-like activity improves growth performance and immunity against Aeromonas hydrophila and Streptococcus iniae in Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol. 2018; 84:695-703. DOI: 10.1016/j.fsi.2018.10.059. View

Liu D, Ge J, Qiao X, Jiang Y, Liu S, Li Y . High-level mucosal and systemic immune responses induced by oral administration with Lactobacillus-expressed porcine epidemic diarrhea virus (PEDV) S1 region combined with Lactobacillus-expressed N protein. Appl Microbiol Biotechnol. 2011; 93(6):2437-46. PMC: 7080084. DOI: 10.1007/s00253-011-3734-0. View

Xue W, Zhao Q, Li P, Zhang R, Lan J, Wang J . Identification and characterization of a novel nanobody against duck hepatitis A virus type 1. Virology. 2018; 528:101-109. DOI: 10.1016/j.virol.2018.12.013. View

Soliman M, Alfajaro M, Lee M, Jeong Y, Kim D, Son K . The prevalence of duck hepatitis A virus types 1 and 3 on Korean duck farms. Arch Virol. 2014; 160(2):493-8. DOI: 10.1007/s00705-014-2264-3. View

Wu X, Li X, Zhang Q, Wulin S, Bai X, Zhang T . Identification of a conserved B-cell epitope on duck hepatitis A type 1 virus VP1 protein. PLoS One. 2015; 10(2):e0118041. PMC: 4337900. DOI: 10.1371/journal.pone.0118041. View

Galdeano C, Cazorla S, Lemme Dumit J, Velez E, Perdigon G . Beneficial Effects of Probiotic Consumption on the Immune System. Ann Nutr Metab. 2019; 74(2):115-124. DOI: 10.1159/000496426. View

Lavelle E, OHagan D . Delivery systems and adjuvants for oral vaccines. Expert Opin Drug Deliv. 2006; 3(6):747-62. DOI: 10.1517/17425247.3.6.747. View

10.

Chen L, Xu Q, Zhang R, Yang L, Li J, Xie Z . Improved duplex RT-PCR assay for differential diagnosis of mixed infection of duck hepatitis A virus type 1 and type 3 in ducklings. J Virol Methods. 2013; 192(1-2):12-7. DOI: 10.1016/j.jviromet.2013.04.012. View

11.

Gao J, Chen J, Si X, Xie Z, Zhu Y, Zhang X . Genetic variation of the VP1 gene of the virulent duck hepatitis A virus type 1 (DHAV-1) isolates in Shandong province of China. Virol Sin. 2012; 27(4):248-53. PMC: 8218140. DOI: 10.1007/s12250-012-3255-8. View

12.

Zou Z, Ma J, Huang K, Chen H, Liu Z, Jin M . Live Attenuated Vaccine Based on Duck Enteritis Virus against Duck Hepatitis A Virus Types 1 and 3. Front Microbiol. 2016; 7:1613. PMC: 5056193. DOI: 10.3389/fmicb.2016.01613. View

13.

Pontes D, de Azevedo M, Chatel J, Langella P, Azevedo V, Miyoshi A . Lactococcus lactis as a live vector: heterologous protein production and DNA delivery systems. Protein Expr Purif. 2011; 79(2):165-75. DOI: 10.1016/j.pep.2011.06.005. View

14.

Matsushita H, Hosoi A, Ueha S, Abe J, Fujieda N, Tomura M . Cytotoxic T lymphocytes block tumor growth both by lytic activity and IFNγ-dependent cell-cycle arrest. Cancer Immunol Res. 2014; 3(1):26-36. DOI: 10.1158/2326-6066.CIR-14-0098. View

15.

Chen J, Zhang R, Lin S, Li P, Lan J, Xie Z . Construction and characterization of an improved DNA-launched infectious clone of duck hepatitis a virus type 1. Virol J. 2017; 14(1):212. PMC: 5670519. DOI: 10.1186/s12985-017-0883-5. View

16.

Cai R, Jiang Y, Yang W, Yang W, Shi S, Shi C . Surface-Displayed IL-10 by Recombinant Lactobacillus plantarum Reduces Th1 Responses of RAW264.7 Cells Stimulated with Poly(I:C) or LPS. J Microbiol Biotechnol. 2015; 26(2):421-31. DOI: 10.4014/jmb.1509.09030. View

17.

Kang M, Roh J, Jang H . Protective efficacy of a bivalent live attenuated vaccine against duck hepatitis A virus types 1 and 3 in ducklings. Vet Microbiol. 2018; 214:108-112. DOI: 10.1016/j.vetmic.2017.12.018. View

18.

Mosmann T, Cherwinski H, Bond M, Giedlin M, Coffman R . Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986; 136(7):2348-57. View

19.

Steidler L, Hans W, SCHOTTE L, Neirynck S, Obermeier F, Falk W . Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science. 2000; 289(5483):1352-5. DOI: 10.1126/science.289.5483.1352. View

20.

Shouval D, Ouahed J, Biswas A, Goettel J, Horwitz B, Klein C . Interleukin 10 receptor signaling: master regulator of intestinal mucosal homeostasis in mice and humans. Adv Immunol. 2014; 122:177-210. PMC: 4741283. DOI: 10.1016/B978-0-12-800267-4.00005-5. View