Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs

Overview

Journal Vet Sci

Publisher MDPI

Date 2025 Jan 24

PMID 39852910

Authors

Yunyun Ma

Junjun Shao

Wei Liu

Shandian Gao

Guangqing Zhou

Xuefeng Qi

Huiyun Chang

Affiliations

Soon will be listed here.

Abstract

The vesicular stomatitis virus (VSV)-vectored African swine fever virus (ASFV) vaccine can induce efficient immune response, but the potential mechanism remains unsolved. In order to investigate the efficacy of recombinant viruses (VSV-p35, VSV-p72)-mediated dendritic cells (DCs) maturation and the mechanism of inducing T-cell immune response, the functional effects of recombinant viruses on DC activation and target antigens presentation were explored in this study. The results showed that surface-marked molecules (CD80, CD86, CD40, and MHC-II) and secreted cytokines (IL-4, TNF-α, IFN-γ) were highly expressed in the recombinant virus-infected DCs. In addition, the co-culture results of recombinant virus-treated DCs with naive T cells showed that the Th1- and Th17-type responses were effectively activated. Taken together, the study indicated that the VSV-vectored ASFV vaccine activated the maturation of DCs and the Th1- and Th17-type immune response, which provided a theoretical basis for the development of novel ASF vaccines.

References

Argilaguet J, Perez-Martin E, Nofrarias M, Gallardo C, Accensi F, Lacasta A . DNA vaccination partially protects against African swine fever virus lethal challenge in the absence of antibodies. PLoS One. 2012; 7(9):e40942. PMC: 3458849. DOI: 10.1371/journal.pone.0040942. View

Lokhandwala S, Waghela S, Bray J, Sangewar N, Charendoff C, Martin C . Adenovirus-vectored novel African Swine Fever Virus antigens elicit robust immune responses in swine. PLoS One. 2017; 12(5):e0177007. PMC: 5421782. DOI: 10.1371/journal.pone.0177007. View

Plevin R, Knoll M, McKay M, Arbabi S, Cuschieri J . The Role of Lipopolysaccharide Structure in Monocyte Activation and Cytokine Secretion. Shock. 2015; 45(1):22-7. PMC: 5181111. DOI: 10.1097/SHK.0000000000000470. View

Pelzel-McCluskey A . Vesicular Stomatitis Virus. Vet Clin North Am Equine Pract. 2023; 39(1):147-155. DOI: 10.1016/j.cveq.2022.11.004. View

Gille C, Steffen F, Lauber K, Keppeler H, Leiber A, Spring B . Clearance of apoptotic neutrophils is diminished in cord blood monocytes and does not lead to reduced IL-8 production. Pediatr Res. 2009; 66(5):507-12. DOI: 10.1203/PDR.0b013e3181b9b470. View

Karalyan Z, Ghonyan S, Poghosyan D, Hakobyan L, Avagyan H, Avetisyan A . Infection of Human Macrophage-Like Cells by African Swine Fever Virus. Front Biosci (Landmark Ed). 2024; 29(4):164. DOI: 10.31083/j.fbl2904164. View

Payne R, Silk S, Elias S, Miura K, Diouf A, Galaway F . Human vaccination against RH5 induces neutralizing antimalarial antibodies that inhibit RH5 invasion complex interactions. JCI Insight. 2017; 2(21). PMC: 5752323. DOI: 10.1172/jci.insight.96381. View

Kaiser J, Nelson C, Liu X, Park H, Matsuoka Y, Luongo C . Mucosal prime-boost immunization with live murine pneumonia virus-vectored SARS-CoV-2 vaccine is protective in macaques. Nat Commun. 2024; 15(1):3553. PMC: 11053155. DOI: 10.1038/s41467-024-47784-6. View

Barderas M, Rodriguez F, Gomez-Puertas P, Aviles M, Beitia F, Alonso C . Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins. Arch Virol. 2001; 146(9):1681-91. DOI: 10.1007/s007050170056. View

10.

Hivroz C, Chemin K, Tourret M, Bohineust A . Crosstalk between T lymphocytes and dendritic cells. Crit Rev Immunol. 2012; 32(2):139-55. DOI: 10.1615/critrevimmunol.v32.i2.30. View

11.

Abdelmageed A, Ferran M . The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus. Curr Protoc Microbiol. 2020; 58(1):e110. PMC: 7449588. DOI: 10.1002/cpmc.110. View

12.

Schulz O, Edwards A, Schito M, Aliberti J, Manickasingham S, Sher A . CD40 triggering of heterodimeric IL-12 p70 production by dendritic cells in vivo requires a microbial priming signal. Immunity. 2000; 13(4):453-62. DOI: 10.1016/s1074-7613(00)00045-5. View

13.

Qiao Y, Zhu M, Xu S, Jiao W, Ni H, Tao Z . Allergen-induced CD11c + dendritic cell pyroptosis aggravates allergic rhinitis. Cell Commun Signal. 2023; 21(1):281. PMC: 10566027. DOI: 10.1186/s12964-023-01309-8. View

14.

Barouch D, Tomaka F, Wegmann F, Stieh D, Alter G, Robb M . Evaluation of a mosaic HIV-1 vaccine in a multicentre, randomised, double-blind, placebo-controlled, phase 1/2a clinical trial (APPROACH) and in rhesus monkeys (NHP 13-19). Lancet. 2018; 392(10143):232-243. PMC: 6192527. DOI: 10.1016/S0140-6736(18)31364-3. View

15.

Lutz M . Induction of CD4(+) Regulatory and Polarized Effector/helper T Cells by Dendritic Cells. Immune Netw. 2016; 16(1):13-25. PMC: 4770096. DOI: 10.4110/in.2016.16.1.13. View

16.

Ma Y, Shao J, Liu W, Gao S, Peng D, Miao C . A vesicular stomatitis virus-based African swine fever vaccine prototype effectively induced robust immune responses in mice following a single-dose immunization. Front Microbiol. 2024; 14:1310333. PMC: 10797088. DOI: 10.3389/fmicb.2023.1310333. View

17.

Breckpot K, Aerts J, Thielemans K . Lentiviral vectors for cancer immunotherapy: transforming infectious particles into therapeutics. Gene Ther. 2007; 14(11):847-62. DOI: 10.1038/sj.gt.3302947. View

18.

Rock D . Thoughts on African Swine Fever Vaccines. Viruses. 2021; 13(5). PMC: 8161283. DOI: 10.3390/v13050943. View

19.

Baden L, Walsh S, Seaman M, Cohen Y, Johnson J, Licona J . First-in-Human Randomized, Controlled Trial of Mosaic HIV-1 Immunogens Delivered via a Modified Vaccinia Ankara Vector. J Infect Dis. 2018; 218(4):633-644. PMC: 6047429. DOI: 10.1093/infdis/jiy212. View

20.

Lina C, Conghua W, Nan L, Ping Z . Combined treatment of etanercept and MTX reverses Th1/Th2, Th17/Treg imbalance in patients with rheumatoid arthritis. J Clin Immunol. 2011; 31(4):596-605. DOI: 10.1007/s10875-011-9542-6. View